Detailed information NTG0849517

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V5F98_RS10650
• Genome accession: NZ_CP145052
• Coordinates: 2010440..2011666 (+)
• Length: 408 a.a.
• NCBI ID: WP_003692100.1
• Uniprot ID: Q5F5L4
• Organism: Neisseria gonorrhoeae strain WHO_F_2024
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2005440..2016666

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V5F98_RS10620 (V5F98_10635)	-	2006472..2006600 (-)	129	WP_256204576.1	hypothetical protein	-
V5F98_RS10625 (V5F98_10640)	dksA	2006615..2007031 (-)	417	WP_003692108.1	RNA polymerase-binding protein DksA	-
V5F98_RS10630 (V5F98_10645)	proC	2007180..2007971 (-)	792	WP_003694504.1	pyrroline-5-carboxylate reductase	-
V5F98_RS10635 (V5F98_10650)	-	2008020..2008427 (-)	408	WP_003690202.1	hypothetical protein	-
V5F98_RS10640 (V5F98_10655)	-	2008427..2009113 (-)	687	WP_003690201.1	YggS family pyridoxal phosphate-dependent enzyme	-
V5F98_RS10645 (V5F98_10660)	pilT	2009233..2010276 (+)	1044	WP_003690200.1	type IV pilus twitching motility protein PilT	Machinery gene
V5F98_RS10650 (V5F98_10665)	pilU	2010440..2011666 (+)	1227	WP_003692100.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
V5F98_RS10655 (V5F98_10670)	yccS	2011783..2013933 (+)	2151	WP_003694501.1	YccS family putative transporter	-
V5F98_RS10660 (V5F98_10675)	-	2014416..2014700 (-)	285	WP_003692093.1	GIY-YIG nuclease family protein	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45698.33 Da
• Isoelectric Point: 7.2909

>NTDB_id=849517 V5F98_RS10650 WP_003692100.1 2010440..2011666(+) (pilU) [Neisseria gonorrhoeae strain WHO_F_2024]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGTHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=849517 V5F98_RS10650 WP_003692100.1 2010440..2011666(+) (pilU) [Neisseria gonorrhoeae strain WHO_F_2024]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCACACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACTACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCGGGAATTGGGCTTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGACCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: Q5F5L4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.875	88.48	0.397
pilU	Pseudomonas stutzeri DSM 10701	45.225	87.255	0.395
pilU	Vibrio cholerae strain A1552	47.024	82.353	0.387