Detailed information NTG0848728

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V5G12_RS10235
• Genome accession: NZ_CP145021
• Coordinates: 1938113..1939339 (-)
• Length: 408 a.a.
• NCBI ID: WP_003697786.1
• Uniprot ID: B4RPS5
• Organism: Neisseria gonorrhoeae strain WHO_V_2024
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1933113..1944339

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V5G12_RS10225 (V5G12_10245)	-	1935078..1935362 (+)	285	WP_003692093.1	GIY-YIG nuclease family protein	-
V5G12_RS10230 (V5G12_10250)	yccS	1935846..1937996 (-)	2151	WP_003697785.1	YccS family putative transporter	-
V5G12_RS10235 (V5G12_10255)	pilU	1938113..1939339 (-)	1227	WP_003697786.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
V5G12_RS10240 (V5G12_10260)	pilT	1939503..1940546 (-)	1044	WP_003690200.1	type IV pilus twitching motility protein PilT	Machinery gene
V5G12_RS10245 (V5G12_10265)	-	1940666..1941352 (+)	687	WP_003699139.1	YggS family pyridoxal phosphate-dependent enzyme	-
V5G12_RS10250 (V5G12_10270)	-	1941352..1941759 (+)	408	WP_003690202.1	hypothetical protein	-
V5G12_RS10255 (V5G12_10275)	proC	1941790..1942599 (+)	810	WP_228547131.1	pyrroline-5-carboxylate reductase	-
V5G12_RS10260 (V5G12_10280)	dksA	1942747..1943163 (+)	417	WP_003692108.1	RNA polymerase-binding protein DksA	-
V5G12_RS10265 (V5G12_10285)	-	1943178..1943306 (+)	129	WP_256204576.1	hypothetical protein	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45668.30 Da
• Isoelectric Point: 7.2909

>NTDB_id=848728 V5G12_RS10235 WP_003697786.1 1938113..1939339(-) (pilU) [Neisseria gonorrhoeae strain WHO_V_2024]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=848728 V5G12_RS10235 WP_003697786.1 1938113..1939339(-) (pilU) [Neisseria gonorrhoeae strain WHO_V_2024]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACTACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCGGGAATTGGGCTTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGACCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: B4RPS5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.875	88.48	0.397
pilU	Pseudomonas stutzeri DSM 10701	45.225	87.255	0.395
pilU	Vibrio cholerae strain A1552	47.024	82.353	0.387