Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QTV53_RS03320 Genome accession   NZ_CP128529
Coordinates   648439..648918 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 132077     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 643439..653918
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QTV53_RS03295 - 644020..644784 (-) 765 WP_006248395.1 TSUP family transporter -
  QTV53_RS03300 proB 644784..645868 (-) 1085 Protein_637 glutamate 5-kinase -
  QTV53_RS03305 folA 645980..646474 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  QTV53_RS03310 - 646594..646824 (+) 231 WP_006248398.1 YgjV family protein -
  QTV53_RS03315 radA 646879..648258 (-) 1380 WP_006248399.1 DNA repair protein RadA -
  QTV53_RS03320 pilA 648439..648918 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  QTV53_RS03325 - 648939..649382 (+) 444 Protein_642 pilus assembly protein PilB -
  QTV53_RS03330 pilC 649388..650584 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  QTV53_RS03335 - 650587..651243 (+) 657 WP_006248404.1 prepilin peptidase -
  QTV53_RS03340 coaE 651279..651914 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  QTV53_RS03345 yacG 651896..652081 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  QTV53_RS03350 holA 652082..653107 (-) 1026 WP_006253426.1 DNA polymerase III subunit delta -
  QTV53_RS03355 lptE 653150..653638 (-) 489 WP_006248408.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=848467 QTV53_RS03320 WP_006248401.1 648439..648918(+) (pilA) [Mannheimia haemolytica strain 132077]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=848467 QTV53_RS03320 WP_006248401.1 648439..648918(+) (pilA) [Mannheimia haemolytica strain 132077]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491