Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QTV61_RS08930 Genome accession   NZ_CP128520
Coordinates   1695174..1695653 (-) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 191452     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1690174..1700653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QTV61_RS08895 lptE 1690454..1690942 (+) 489 WP_006248408.1 LPS assembly lipoprotein LptE -
  QTV61_RS08900 holA 1690985..1692010 (+) 1026 WP_006253426.1 DNA polymerase III subunit delta -
  QTV61_RS08905 yacG 1692011..1692196 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  QTV61_RS08910 coaE 1692178..1692813 (-) 636 WP_006248405.1 dephospho-CoA kinase -
  QTV61_RS08915 - 1692849..1693505 (-) 657 WP_006248404.1 prepilin peptidase -
  QTV61_RS08920 pilC 1693508..1694704 (-) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  QTV61_RS08925 - 1694710..1695153 (-) 444 Protein_1726 pilus assembly protein PilB -
  QTV61_RS08930 pilA 1695174..1695653 (-) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  QTV61_RS08935 radA 1695834..1697213 (+) 1380 WP_006248399.1 DNA repair protein RadA -
  QTV61_RS08940 - 1697268..1697498 (-) 231 WP_006248398.1 YgjV family protein -
  QTV61_RS08945 folA 1697618..1698112 (-) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  QTV61_RS08950 proB 1698224..1699308 (+) 1085 Protein_1731 glutamate 5-kinase -
  QTV61_RS08955 - 1699308..1700072 (+) 765 WP_006248395.1 TSUP family transporter -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=848289 QTV61_RS08930 WP_006248401.1 1695174..1695653(-) (pilA) [Mannheimia haemolytica strain 191452]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=848289 QTV61_RS08930 WP_006248401.1 1695174..1695653(-) (pilA) [Mannheimia haemolytica strain 191452]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491