Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QTV60_RS09220 Genome accession   NZ_CP128518
Coordinates   1753133..1753612 (-) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 192037     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1748133..1758612
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QTV60_RS09185 lptE 1748413..1748901 (+) 489 WP_006248408.1 LPS assembly lipoprotein LptE -
  QTV60_RS09190 holA 1748944..1749969 (+) 1026 WP_006253426.1 DNA polymerase III subunit delta -
  QTV60_RS09195 yacG 1749970..1750155 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  QTV60_RS09200 coaE 1750137..1750772 (-) 636 WP_006248405.1 dephospho-CoA kinase -
  QTV60_RS09205 - 1750808..1751464 (-) 657 WP_006248404.1 prepilin peptidase -
  QTV60_RS09210 pilC 1751467..1752663 (-) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  QTV60_RS09215 - 1752669..1753112 (-) 444 Protein_1778 pilus assembly protein PilB -
  QTV60_RS09220 pilA 1753133..1753612 (-) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  QTV60_RS09225 radA 1753793..1755172 (+) 1380 WP_006248399.1 DNA repair protein RadA -
  QTV60_RS09230 - 1755227..1755457 (-) 231 WP_006248398.1 YgjV family protein -
  QTV60_RS09235 folA 1755577..1756071 (-) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  QTV60_RS09240 proB 1756183..1757267 (+) 1085 Protein_1783 glutamate 5-kinase -
  QTV60_RS09245 - 1757267..1758031 (+) 765 WP_006248395.1 TSUP family transporter -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=848244 QTV60_RS09220 WP_006248401.1 1753133..1753612(-) (pilA) [Mannheimia haemolytica strain 192037]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=848244 QTV60_RS09220 WP_006248401.1 1753133..1753612(-) (pilA) [Mannheimia haemolytica strain 192037]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491