Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   QSJ10_RS10235 Genome accession   NZ_CP128494
Coordinates   1883409..1884482 (-) Length   357 a.a.
NCBI ID   WP_011231917.1    Uniprot ID   Q5KX66
Organism   Geobacillus stearothermophilus ATCC 12980     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1878409..1889482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QSJ10_RS10195 (QSJ10_10195) - 1879175..1879969 (+) 795 WP_033016406.1 YqhG family protein -
  QSJ10_RS10200 (QSJ10_10200) - 1880066..1880245 (-) 180 WP_033016405.1 YqzE family protein -
  QSJ10_RS10205 (QSJ10_10205) comGG 1880386..1880775 (-) 390 WP_033016402.1 competence type IV pilus minor pilin ComGG -
  QSJ10_RS10210 (QSJ10_10210) comGF 1880884..1881285 (-) 402 WP_230581369.1 competence type IV pilus minor pilin ComGF -
  QSJ10_RS10215 (QSJ10_10215) - 1881309..1881635 (-) 327 WP_033016399.1 hypothetical protein -
  QSJ10_RS10220 (QSJ10_10220) comGD 1881619..1882062 (-) 444 WP_227412606.1 competence type IV pilus minor pilin ComGD -
  QSJ10_RS10225 (QSJ10_10225) comGC 1882046..1882342 (-) 297 WP_080706542.1 competence type IV pilus major pilin ComGC Machinery gene
  QSJ10_RS10230 (QSJ10_10230) comGB 1882384..1883412 (-) 1029 WP_033016395.1 competence type IV pilus assembly protein ComGB -
  QSJ10_RS10235 (QSJ10_10235) comGA 1883409..1884482 (-) 1074 WP_011231917.1 competence type IV pilus ATPase ComGA Machinery gene
  QSJ10_RS10240 (QSJ10_10240) - 1884675..1885373 (+) 699 WP_011231918.1 helix-turn-helix domain-containing protein -
  QSJ10_RS10245 (QSJ10_10245) - 1885530..1885772 (+) 243 WP_033010198.1 DUF2626 domain-containing protein -
  QSJ10_RS10250 (QSJ10_10250) - 1885807..1886932 (-) 1126 Protein_1946 SAM-dependent methyltransferase -
  QSJ10_RS10255 (QSJ10_10255) - 1887412..1888044 (-) 633 WP_049625174.1 MBL fold metallo-hydrolase -
  QSJ10_RS10260 (QSJ10_10260) - 1888223..1888399 (+) 177 WP_033010201.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39570.00 Da        Isoelectric Point: 8.7901

>NTDB_id=847696 QSJ10_RS10235 WP_011231917.1 1883409..1884482(-) (comGA) [Geobacillus stearothermophilus ATCC 12980]
MLNEIEQTANRLLAEAVQRRASDLHLVPRRRDAAVRLRLDGMLVDVGVLSKETAERLIAHFKFLAGMDIGERRRPQSGAM
EVAEFGETVYLRLSTLPTLYDESLVIRLLPQRFSLPLRELSLFFRSTARLFSFMQHPQGLVLLTGPTGSGKTTTLYTLLD
LCQAERQRNIITLEDPIEKQNERLLQVQINEKAGITYAAGLKAALRHDPDVLMVGEIRDHDTAAIAIRSALSGHLVVSTM
HAADAVGAVYRLHEFGIPLGDLAETLLAVSAQRLVELCCPLCGDDCHPSCGRLGRRRRTAVYELLCGSALEDVIRSLSNG
RGKPKRSYMTLARLIRKGIALGYLPVRMLELVGGGER

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=847696 QSJ10_RS10235 WP_011231917.1 1883409..1884482(-) (comGA) [Geobacillus stearothermophilus ATCC 12980]
GTGCTGAACGAGATCGAACAAACGGCAAACCGCCTTCTCGCCGAAGCGGTGCAGCGCCGCGCCTCGGATCTTCACCTCGT
TCCGCGCCGCCGCGATGCCGCTGTTCGTCTTCGGCTTGATGGCATGCTTGTCGACGTTGGCGTGCTTTCGAAGGAAACGG
CGGAACGTCTCATCGCCCACTTTAAATTTTTAGCCGGCATGGACATCGGGGAACGACGCCGTCCGCAAAGCGGCGCCATG
GAAGTGGCTGAGTTTGGGGAAACGGTGTATTTGCGTTTATCGACATTGCCGACGCTCTACGATGAAAGCCTCGTCATTCG
GCTTCTGCCGCAGCGCTTCTCTCTGCCGCTTCGCGAACTATCTTTATTCTTCCGCTCCACCGCACGATTATTTTCCTTCA
TGCAGCATCCGCAAGGGCTTGTGCTGTTGACCGGCCCGACGGGGTCGGGAAAAACGACGACCCTCTACACCCTTCTTGAT
CTTTGCCAAGCTGAAAGGCAACGCAACATCATCACGCTTGAAGATCCGATCGAAAAACAGAACGAACGATTGTTGCAAGT
GCAAATCAATGAAAAAGCAGGCATCACATACGCCGCCGGGTTAAAAGCGGCGCTGCGCCATGACCCGGATGTGTTGATGG
TCGGCGAGATCCGCGATCATGATACGGCGGCCATTGCCATCCGTTCAGCGTTGAGCGGCCATTTGGTCGTCTCGACGATG
CACGCCGCCGATGCAGTCGGCGCCGTCTACCGGCTGCATGAATTCGGCATTCCGCTCGGCGATTTGGCCGAGACGCTGCT
TGCCGTCTCTGCCCAGCGGCTTGTCGAGCTTTGCTGCCCGCTGTGCGGCGACGATTGCCATCCATCGTGCGGCCGTCTTG
GACGACGCCGGCGCACGGCTGTTTATGAGTTGCTTTGCGGTTCAGCGCTGGAGGACGTGATTCGTTCCCTCTCCAATGGG
AGAGGGAAGCCAAAGCGCTCTTATATGACGCTGGCGCGCTTGATCCGCAAGGGGATTGCGCTCGGCTACCTGCCCGTTCG
CATGCTTGAGCTCGTCGGAGGGGGAGAAAGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5KX66

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

50.725

96.639

0.49