Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   V4941_RS17540 Genome accession   NZ_CP144736
Coordinates   3911019..3911639 (+) Length   206 a.a.
NCBI ID   WP_012703009.1    Uniprot ID   C1DHQ9
Organism   Azotobacter vinelandii DJ     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3906019..3916639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4941_RS17525 (V4941_17520) - 3906657..3909116 (-) 2460 WP_012703012.1 penicillin-binding protein 1A -
  V4941_RS17530 (V4941_17525) pilM 3909298..3910362 (+) 1065 WP_041807416.1 pilus assembly protein PilM Machinery gene
  V4941_RS17535 (V4941_17530) - 3910363..3911022 (+) 660 WP_012703010.1 PilN domain-containing protein -
  V4941_RS17540 (V4941_17535) pilO 3911019..3911639 (+) 621 WP_012703009.1 type 4a pilus biogenesis protein PilO Machinery gene
  V4941_RS17545 (V4941_17540) - 3911636..3912160 (+) 525 WP_012703008.1 pilus assembly protein PilP -
  V4941_RS17550 (V4941_17545) pilQ 3912214..3914313 (+) 2100 WP_175555950.1 type IV pilus secretin PilQ Machinery gene
  V4941_RS17555 (V4941_17550) aroK 3914318..3914836 (+) 519 WP_012703006.1 shikimate kinase AroK -
  V4941_RS17560 (V4941_17555) aroB 3914888..3915991 (+) 1104 WP_012703005.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 206 a.a.        Molecular weight: 22929.44 Da        Isoelectric Point: 5.0972

>NTDB_id=847655 V4941_RS17540 WP_012703009.1 3911019..3911639(+) (pilO) [Azotobacter vinelandii DJ]
MSLSGSLESLRKIDLADLDLDNLGSWPAPLRTATCLLMLIVVLALGYNFHLKDLQAQLERERGEEGALKQQFSTKAFQAA
NLEAYRQQMAEMEASFGALLRQLPSDTEVPALLDDITRTGLGSGLEFEEIKLLDEVVRQFYIELPIQISVMGAYHDLATF
VSGAASLPRIVTLHDFEIKPVASGNTKLRMKILAKTYRYNDKGPQK

Nucleotide


Download         Length: 621 bp        

>NTDB_id=847655 V4941_RS17540 WP_012703009.1 3911019..3911639(+) (pilO) [Azotobacter vinelandii DJ]
ATGAGCCTGTCCGGGTCCCTGGAGAGTCTGCGCAAGATCGATCTGGCCGATCTGGATCTGGACAACCTCGGCTCGTGGCC
GGCTCCGCTGAGGACCGCCACCTGCCTGCTGATGCTGATCGTTGTTCTGGCGCTGGGCTACAACTTCCATCTCAAGGATC
TGCAGGCGCAGCTCGAGCGGGAGCGGGGCGAAGAGGGGGCCTTGAAGCAGCAGTTCTCCACCAAGGCTTTCCAGGCCGCG
AACCTGGAAGCCTACAGGCAGCAGATGGCGGAAATGGAGGCCTCTTTCGGCGCCTTGTTGCGGCAATTGCCGAGCGATAC
CGAGGTGCCCGCTCTGCTCGATGACATCACCCGTACCGGACTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAACTTCTCG
ACGAAGTTGTCCGGCAGTTCTATATCGAATTGCCGATCCAAATATCCGTAATGGGTGCTTATCACGATCTGGCTACTTTC
GTAAGTGGCGCCGCCAGTCTTCCACGCATCGTCACATTGCATGATTTCGAAATAAAACCAGTGGCTTCGGGAAATACGAA
GTTGCGCATGAAGATATTGGCCAAGACCTATCGCTATAACGACAAGGGACCGCAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C1DHQ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.078

93.689

0.422
  pilO Acinetobacter baumannii D1279779

40.642

90.777

0.369
  comO/pilO Acinetobacter baylyi ADP1

40.86

90.291

0.369