Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   V4939_RS20320 Genome accession   NZ_CP144733
Coordinates   4417396..4418016 (-) Length   206 a.a.
NCBI ID   WP_012703009.1    Uniprot ID   C1DHQ9
Organism   Azotobacter vinelandii DJ     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4412396..4423016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4939_RS20300 (V4939_20300) aroB 4413044..4414147 (-) 1104 WP_012703005.1 3-dehydroquinate synthase -
  V4939_RS20305 (V4939_20305) aroK 4414199..4414717 (-) 519 WP_012703006.1 shikimate kinase AroK -
  V4939_RS20310 (V4939_20310) pilQ 4414722..4416821 (-) 2100 WP_175555950.1 type IV pilus secretin PilQ Machinery gene
  V4939_RS20315 (V4939_20315) - 4416875..4417399 (-) 525 WP_012703008.1 pilus assembly protein PilP -
  V4939_RS20320 (V4939_20320) pilO 4417396..4418016 (-) 621 WP_012703009.1 type 4a pilus biogenesis protein PilO Machinery gene
  V4939_RS20325 (V4939_20325) - 4418013..4418672 (-) 660 WP_012703010.1 PilN domain-containing protein -
  V4939_RS20330 (V4939_20330) pilM 4418673..4419737 (-) 1065 WP_041807416.1 pilus assembly protein PilM Machinery gene
  V4939_RS20335 (V4939_20335) - 4419919..4422378 (+) 2460 WP_012703012.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 206 a.a.        Molecular weight: 22929.44 Da        Isoelectric Point: 5.0972

>NTDB_id=847569 V4939_RS20320 WP_012703009.1 4417396..4418016(-) (pilO) [Azotobacter vinelandii DJ]
MSLSGSLESLRKIDLADLDLDNLGSWPAPLRTATCLLMLIVVLALGYNFHLKDLQAQLERERGEEGALKQQFSTKAFQAA
NLEAYRQQMAEMEASFGALLRQLPSDTEVPALLDDITRTGLGSGLEFEEIKLLDEVVRQFYIELPIQISVMGAYHDLATF
VSGAASLPRIVTLHDFEIKPVASGNTKLRMKILAKTYRYNDKGPQK

Nucleotide


Download         Length: 621 bp        

>NTDB_id=847569 V4939_RS20320 WP_012703009.1 4417396..4418016(-) (pilO) [Azotobacter vinelandii DJ]
ATGAGCCTGTCCGGGTCCCTGGAGAGTCTGCGCAAGATCGATCTGGCCGATCTGGATCTGGACAACCTCGGCTCGTGGCC
GGCTCCGCTGAGGACCGCCACCTGCCTGCTGATGCTGATCGTTGTTCTGGCGCTGGGCTACAACTTCCATCTCAAGGATC
TGCAGGCGCAGCTCGAGCGGGAGCGGGGCGAAGAGGGGGCCTTGAAGCAGCAGTTCTCCACCAAGGCTTTCCAGGCCGCG
AACCTGGAAGCCTACAGGCAGCAGATGGCGGAAATGGAGGCCTCTTTCGGCGCCTTGTTGCGGCAATTGCCGAGCGATAC
CGAGGTGCCCGCTCTGCTCGATGACATCACCCGTACCGGACTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAACTTCTCG
ACGAAGTTGTCCGGCAGTTCTATATCGAATTGCCGATCCAAATATCCGTAATGGGTGCTTATCACGATCTGGCTACTTTC
GTAAGTGGCGCCGCCAGTCTTCCACGCATCGTCACATTGCATGATTTCGAAATAAAACCAGTGGCTTCGGGAAATACGAA
GTTGCGCATGAAGATATTGGCCAAGACCTATCGCTATAACGACAAGGGACCGCAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C1DHQ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.078

93.689

0.422
  pilO Acinetobacter baumannii D1279779

40.642

90.777

0.369
  comO/pilO Acinetobacter baylyi ADP1

40.86

90.291

0.369