Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QP609_RS04845 Genome accession   NZ_CP128404
Coordinates   1053609..1054772 (+) Length   387 a.a.
NCBI ID   WP_042358565.1    Uniprot ID   A0A6N7NYV7
Organism   Stenotrophomonas sp. 8(2023)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1048609..1059772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QP609_RS04815 soxR 1048745..1049179 (+) 435 WP_021202603.1 redox-sensitive transcriptional activator SoxR -
  QP609_RS04820 - 1049252..1049854 (-) 603 WP_014646214.1 short chain dehydrogenase -
  QP609_RS04825 - 1049953..1050828 (+) 876 WP_032958506.1 LysR family transcriptional regulator -
  QP609_RS04830 proC 1050888..1051709 (-) 822 WP_014646216.1 pyrroline-5-carboxylate reductase -
  QP609_RS04835 - 1051728..1052405 (-) 678 WP_032958502.1 YggS family pyridoxal phosphate-dependent enzyme -
  QP609_RS04840 pilT 1052493..1053530 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  QP609_RS04845 pilU 1053609..1054772 (+) 1164 WP_042358565.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QP609_RS04850 - 1054894..1055877 (+) 984 WP_049405029.1 aldo/keto reductase -
  QP609_RS04855 - 1055940..1056836 (-) 897 WP_014646219.1 LysR family transcriptional regulator -
  QP609_RS04860 - 1057135..1057347 (+) 213 WP_014646220.1 DUF4287 domain-containing protein -
  QP609_RS04865 - 1057458..1058165 (+) 708 WP_014036228.1 YitT family protein -
  QP609_RS04870 - 1058226..1058771 (-) 546 WP_197620919.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43108.51 Da        Isoelectric Point: 6.5899

>NTDB_id=847249 QP609_RS04845 WP_042358565.1 1053609..1054772(+) (pilU) [Stenotrophomonas sp. 8(2023)]
MPSWEPAVNSTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKT
HECNFAIGLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGY
RNQNSTGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLC
TLHANNANQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVM
KDSVQLGMKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=847249 QP609_RS04845 WP_042358565.1 1053609..1054772(+) (pilU) [Stenotrophomonas sp. 8(2023)]
ATGCCCTCTTGGGAGCCCGCCGTGAACAGCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCA
GCGCGCCTCGGACCTGTTCATCACGGCCGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACCCAGA
CCCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACG
CACGAATGCAACTTCGCCATCGGCCTGTCCGGCGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGG
CATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACCGTGGAAGAACTGAGCCTGCCGCCGATCATCAAGACGCTGGCGA
TGACCAAGCGCGGCATCATCCTGTTCGTCGGCGCCACCGGTACCGGTAAATCGACCTCGCTGGCAGCGATGATCGGTTAC
CGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCAT
CACCCAGCGCGAGGTCGGCATCGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGA
TCATGATCGGCGAGGTGCGTACCCGCGAAGGCATGGATCACGCCATCGCGTTCGCCGAAACCGGCCACCTGGTGCTGTGC
ACCCTGCATGCGAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGCAACCAGCTGCT
GATGGACCTGTCGCTGAATCTCAAGGGCGTGGTCGCGCAACAGCTGGTGCCATCGCCCGATGGCCGCTCGCGCAAGGTGG
CGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATG
AAGGACTCGGTCCAGCTTGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGA
GGACGCGCTGCGCTACGCCGATTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCACGTACCC
TGTCGCAGGGGCTGGACGGCGTGGAGATCTCGGAGATCCGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N7NYV7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

94.832

0.643
  pilU Acinetobacter baylyi ADP1

63.585

92.248

0.587
  pilU Vibrio cholerae strain A1552

53.371

91.99

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.099

91.731

0.395