Detailed information NTG0846536

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: QRA13_RS19485
• Genome accession: NZ_CP128184
• Coordinates: 3945390..3946004 (+)
• Length: 204 a.a.
• NCBI ID: WP_045208286.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain YJ0-1 isolate soil
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3940390..3951004

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QRA13_RS19450 (QRA13_19450)	aroE	3941077..3941919 (+)	843	WP_032873946.1	shikimate dehydrogenase	-
QRA13_RS19455 (QRA13_19455)	yhbY	3941913..3942203 (+)	291	WP_045208288.1	ribosome assembly RNA-binding protein YhbY	-
QRA13_RS19460 (QRA13_19460)	-	3942214..3942783 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
QRA13_RS19465 (QRA13_19465)	yqeK	3942773..3943333 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
QRA13_RS19470 (QRA13_19470)	rsfS	3943351..3943707 (+)	357	WP_007408260.1	ribosome silencing factor	-
QRA13_RS19475 (QRA13_19475)	-	3943704..3944441 (+)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
QRA13_RS19480 (QRA13_19480)	comER	3944510..3945331 (-)	822	WP_012118027.1	late competence protein ComER	-
QRA13_RS19485 (QRA13_19485)	comEA	3945390..3946004 (+)	615	WP_045208286.1	helix-hairpin-helix domain-containing protein	Machinery gene
QRA13_RS19490 (QRA13_19490)	-	3946071..3946640 (+)	570	WP_003152868.1	ComE operon protein 2	-
QRA13_RS19495 (QRA13_19495)	comEC	3946641..3948992 (+)	2352	WP_045208284.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
QRA13_RS19500 (QRA13_19500)	-	3949011..3949145 (-)	135	WP_003152870.1	YqzM family protein	-
QRA13_RS19505 (QRA13_19505)	-	3949186..3949337 (+)	152	Protein_3790	hypothetical protein	-
QRA13_RS19510 (QRA13_19510)	holA	3949377..3950418 (+)	1042	Protein_3791	DNA polymerase III subunit delta	-
QRA13_RS19515 (QRA13_19515)	rpsT	3950435..3950701 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22323.60 Da
• Isoelectric Point: 7.7754

>NTDB_id=846536 QRA13_RS19485 WP_045208286.1 3945390..3946004(+) (comEA) [Bacillus velezensis strain YJ0-1 isolate soil]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTTDDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELKVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQVISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=846536 QRA13_RS19485 WP_045208286.1 3945390..3946004(+) (comEA) [Bacillus velezensis strain YJ0-1 isolate soil]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCACAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGTCTGAAGAAAAAGGCGGATGAATTGAAAGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGTGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.115	93.627	0.544
comEA	Lactococcus lactis subsp. cremoris KW2	33.784	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.071	100	0.363