Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   V2198_RS06745 Genome accession   NZ_CP143900
Coordinates   1419365..1419988 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain PA19_NI3_44.22_ST207     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1414365..1424988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V2198_RS06730 (V2198_06730) - 1415052..1417523 (-) 2472 WP_003095841.1 penicillin-binding protein 1A -
  V2198_RS06735 (V2198_06735) pilM 1417708..1418772 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  V2198_RS06740 (V2198_06740) pilN 1418772..1419368 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  V2198_RS06745 (V2198_06745) pilO 1419365..1419988 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  V2198_RS06750 (V2198_06750) comL/pilP 1419985..1420509 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  V2198_RS06755 (V2198_06755) pilQ 1420563..1422707 (+) 2145 WP_033995309.1 type 4a pilus secretin PilQ Machinery gene
  V2198_RS06760 (V2198_06760) aroK 1422719..1423237 (+) 519 WP_003095833.1 shikimate kinase AroK -
  V2198_RS06765 (V2198_06765) aroB 1423287..1424393 (+) 1107 WP_009314395.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=844963 V2198_RS06745 WP_003103268.1 1419365..1419988(+) (pilO) [Pseudomonas aeruginosa strain PA19_NI3_44.22_ST207]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=844963 V2198_RS06745 WP_003103268.1 1419365..1419988(+) (pilO) [Pseudomonas aeruginosa strain PA19_NI3_44.22_ST207]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401