Detailed information NTG0084445

Bioinformatically predicted

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: JMH49_RS07465
• Genome accession: NZ_AP024270
• Coordinates: 1414703..1416976 (+)
• Length: 757 a.a.
• NCBI ID: WP_201350821.1
• Uniprot ID: A0A7R7YJI5
• Organism: Thermus thermophilus strain HB5018
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1409703..1421976

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JMH49_RS07435 (TthHB5018_14740)	-	1409827..1410318 (+)	492	WP_011173430.1	DUF5317 domain-containing protein	-
JMH49_RS07440 (TthHB5018_14750)	-	1410373..1411377 (+)	1005	WP_011173431.1	homoisocitrate dehydrogenase	-
JMH49_RS07445 (TthHB5018_14760)	pilM	1411511..1412644 (+)	1134	WP_201350819.1	type IV pilus assembly protein PilM	Machinery gene
JMH49_RS07450 (TthHB5018_14770)	pilN	1412637..1413260 (+)	624	WP_011173433.1	competence protein	Machinery gene
JMH49_RS07455 (TthHB5018_14780)	pilO	1413250..1413831 (+)	582	WP_011173434.1	type 4a pilus biogenesis protein PilO	Machinery gene
JMH49_RS07460 (TthHB5018_14790)	pilW	1413828..1414706 (+)	879	WP_201350820.1	competence protein	Machinery gene
JMH49_RS07465 (TthHB5018_14800)	pilQ	1414703..1416976 (+)	2274	WP_201350821.1	secretin N-terminal domain-containing protein	Machinery gene
JMH49_RS07470 (TthHB5018_14810)	aroC	1417035..1418186 (+)	1152	WP_011228640.1	chorismate synthase	-
JMH49_RS07475 (TthHB5018_14820)	-	1418211..1418765 (+)	555	WP_011173438.1	shikimate kinase	-
JMH49_RS07480 (TthHB5018_14830)	-	1418743..1419789 (+)	1047	WP_201350822.1	3-dehydroquinate synthase family protein	-
JMH49_RS07485 (TthHB5018_14840)	rsmF	1419835..1421205 (-)	1371	WP_041443492.1	16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF	-
JMH49_RS07490 (TthHB5018_14850)	-	1421258..1421635 (+)	378	WP_011173441.1	4'-phosphopantetheinyl transferase superfamily protein	-
JMH49_RS07495 (TthHB5018_14860)	-	1421613..1421966 (-)	354	WP_201350823.1	DUF4870 domain-containing protein	-

Sequence

Protein

• Length: 757 a.a.
• Molecular weight: 82672.75 Da
• Isoelectric Point: 5.1377

>NTDB_id=84445 JMH49_RS07465 WP_201350821.1 1414703..1416976(+) (pilQ) [Thermus thermophilus strain HB5018]
MKSAWIRAAVIALAGLGAFALAGSFPEEPRFQAPVNLKVSESQVKAGQTLPLDVVLEALARSVGLQPLIYRAYDPSGDPA
KAQPPLPNIKLDFQGKPFREVWDLLFATYGNQYSLDYLFLPPDVVVVAPTQVITALVDAPSRTGAMERRPYIVGIPEIAY
KRTETDAQGQPRTVVNIEGAKAWVQNDLLPFLSREAAGLNVNWIVVEEGGRLKAVLSVLATPEQHARFSDILQRAGIDFR
PLPALAQPKPRVEKTYTLTYATFPELLAFLQSRLPEAQISVVPTNPQRAIVLATEEDHARLSELLKTADVPKTVRRVYAL
QNLTFAEAQERLKPLLEKDLKGARLESLPGNPKALLLEAPEAEHALFAEILKALDVPPQAPEAPQEATLRRLYPLHYANA
EQVAPFLAREVPGIVVQTVPGQPVLSVRGTEKQLAEVESLLAQIDRAPEQGPPVFQRAYQLSNAKAVELAQVLQEALKAR
QAQNQGQQNQALPTREATVVADPRTNTLIVTGTQEDLALVEGLIPKLDQPVPQVSLRVRIQEVQSNLTRSLGLKWNSIAG
GNVAASILDSGLSLIFDSTRSLAALNIMATLDALQQQGLSRALRDVNQTVLNNQTARLQSGETFFIRRVVNDQVERVPFD
VGLIVEVTPQITADGQILLNIKAEISGNVQRNPVDGDVDRFTKQVVTTTLRVKDGETVVLGGLTSQESNQSQQGVPLLMD
IPLIGELFKQRTNESTDKELLVVITADILKETASANP

Nucleotide

• Length: 2274 bp

>NTDB_id=84445 JMH49_RS07465 WP_201350821.1 1414703..1416976(+) (pilQ) [Thermus thermophilus strain HB5018]
ATGAAGAGCGCGTGGATCCGTGCGGCCGTGATCGCCCTCGCAGGGCTAGGGGCTTTTGCCCTTGCGGGGAGCTTTCCCGA
GGAGCCCCGCTTCCAGGCCCCGGTGAACCTCAAGGTCTCGGAATCCCAGGTCAAGGCGGGCCAGACCCTGCCCCTGGACG
TGGTCCTCGAGGCCCTGGCAAGGAGCGTGGGGCTCCAGCCCCTCATCTACCGGGCCTACGACCCAAGCGGGGACCCCGCC
AAGGCCCAGCCCCCCCTGCCCAACATCAAGCTGGACTTCCAGGGCAAGCCCTTCCGGGAGGTCTGGGACCTCCTCTTCGC
CACCTACGGCAACCAGTACAGCCTGGACTACCTCTTCCTGCCCCCCGACGTGGTGGTGGTGGCCCCCACCCAGGTGATCA
CCGCCCTGGTGGACGCCCCGAGCCGCACGGGGGCCATGGAGCGTAGGCCCTACATCGTGGGCATCCCCGAGATCGCCTAC
AAGCGCACGGAGACGGACGCCCAGGGCCAGCCCCGCACGGTCGTCAACATTGAGGGCGCCAAGGCCTGGGTCCAGAACGA
CCTCCTCCCCTTCCTCTCCCGGGAGGCGGCAGGGCTCAACGTGAACTGGATCGTGGTGGAGGAGGGCGGGAGGCTCAAGG
CCGTTCTCTCCGTTCTCGCCACCCCCGAGCAGCACGCCCGCTTCTCCGATATCCTGCAACGGGCCGGGATTGACTTCCGC
CCCCTCCCCGCCCTGGCCCAGCCCAAGCCCCGGGTGGAGAAGACCTACACCCTCACCTACGCCACCTTCCCCGAACTCCT
CGCCTTCCTCCAGTCCCGCCTCCCCGAGGCCCAGATCAGCGTGGTCCCCACCAACCCCCAAAGGGCCATCGTCCTCGCCA
CCGAGGAGGACCACGCCCGCTTGAGCGAGCTTCTGAAGACGGCCGACGTCCCCAAGACCGTCCGCCGGGTCTACGCCCTG
CAGAACCTCACCTTCGCTGAGGCCCAGGAGCGCCTAAAGCCCCTCCTGGAGAAGGACCTCAAGGGGGCCCGCCTGGAAAG
CCTCCCCGGCAACCCCAAGGCCCTCCTCCTCGAGGCCCCCGAGGCGGAGCACGCCCTCTTCGCCGAGATCCTGAAGGCCC
TGGACGTCCCCCCCCAGGCCCCCGAAGCCCCCCAGGAGGCCACCTTGCGGCGCCTCTACCCCCTGCACTACGCCAACGCC
GAGCAGGTGGCCCCCTTCCTCGCCCGGGAGGTGCCGGGGATCGTGGTCCAGACCGTTCCCGGCCAGCCCGTCCTCTCGGT
GCGGGGCACGGAGAAGCAGCTCGCCGAGGTGGAAAGCCTCCTCGCCCAGATTGACCGGGCCCCCGAGCAAGGCCCTCCCG
TGTTCCAGCGGGCCTACCAGCTCTCCAACGCCAAGGCGGTGGAGCTCGCCCAGGTGCTCCAGGAGGCGCTCAAGGCCCGG
CAGGCCCAAAACCAGGGCCAGCAGAACCAGGCCCTCCCCACCCGGGAGGCCACGGTGGTGGCCGACCCCAGGACCAACAC
CCTCATCGTCACCGGCACCCAGGAGGACCTCGCCCTGGTGGAGGGCCTCATCCCCAAGCTGGACCAGCCCGTGCCCCAGG
TTTCCTTGCGGGTGCGCATCCAGGAGGTCCAGTCCAACCTGACCCGTAGCCTCGGCCTCAAGTGGAACAGCATCGCCGGC
GGCAACGTGGCGGCCAGCATCCTGGACTCGGGCCTTTCCCTCATCTTTGACTCCACGCGAAGCCTCGCCGCCCTCAACAT
CATGGCCACCCTGGACGCCCTGCAGCAACAGGGCCTCTCCCGGGCCCTCCGGGACGTGAACCAGACCGTCCTCAACAACC
AGACCGCCCGCCTGCAGTCCGGCGAGACCTTCTTCATCCGGCGGGTGGTGAACGACCAGGTGGAACGGGTCCCCTTTGAC
GTGGGGCTCATCGTGGAGGTCACGCCCCAGATCACCGCCGACGGCCAGATCCTCCTCAACATCAAGGCCGAGATCTCGGG
GAACGTCCAGCGCAACCCCGTGGACGGGGACGTGGACCGCTTCACCAAGCAGGTGGTGACCACCACCCTCAGGGTCAAAG
ACGGGGAGACCGTGGTCCTCGGGGGCCTCACCTCCCAGGAGTCCAACCAGAGCCAGCAGGGAGTGCCCCTCCTCATGGAC
ATCCCCCTGATCGGCGAGCTCTTCAAGCAGCGGACGAACGAGAGCACCGACAAGGAGCTTTTGGTGGTGATCACCGCCGA
CATCCTCAAGGAGACGGCCTCGGCGAACCCCTAA

Domains

Predicted by InterproScan.

• Bacterial type II and III secretion system protein (Secretin) (593-750)

• Bacterial type II/III secretion system short domain (Secretin_N) (391-450)

• Bacterial type II/III secretion system short domain (Secretin_N) (457-533)

3D structure

• AlphaFold DB: A0A7R7YJI5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Thermus thermophilus HB27	98.018	100	0.98