Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   V1232_RS03470 Genome accession   NZ_CP143357
Coordinates   735459..736082 (+) Length   207 a.a.
NCBI ID   WP_003116135.1    Uniprot ID   A0A0H2ZIF6
Organism   Pseudomonas aeruginosa strain 2023CK-01621     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 730459..741082
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1232_RS03455 (V1232_03455) - 731146..733617 (-) 2472 WP_023435970.1 penicillin-binding protein 1A -
  V1232_RS03460 (V1232_03460) pilM 733802..734866 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  V1232_RS03465 (V1232_03465) pilN 734866..735462 (+) 597 WP_023435969.1 type 4a pilus biogenesis protein PilN -
  V1232_RS03470 (V1232_03470) pilO 735459..736082 (+) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO Machinery gene
  V1232_RS03475 (V1232_03475) pilP 736079..736603 (+) 525 WP_023435968.1 type 4a pilus biogenesis lipoprotein PilP -
  V1232_RS03480 (V1232_03480) pilQ 736657..738789 (+) 2133 WP_023435967.1 type 4a pilus secretin PilQ Machinery gene
  V1232_RS03485 (V1232_03485) aroK 738801..739319 (+) 519 WP_003095833.1 shikimate kinase AroK -
  V1232_RS03490 (V1232_03490) aroB 739369..740475 (+) 1107 WP_003112148.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22958.35 Da        Isoelectric Point: 4.9107

>NTDB_id=843576 V1232_RS03470 WP_003116135.1 735459..736082(+) (pilO) [Pseudomonas aeruginosa strain 2023CK-01621]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLRDLQTQLEQQAAQEESLKQQFATKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPASPDNTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=843576 V1232_RS03470 WP_003116135.1 735459..736082(+) (pilO) [Pseudomonas aeruginosa strain 2023CK-01621]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTCCGTGACT
TGCAAACACAGCTCGAACAGCAGGCTGCGCAAGAAGAATCGCTCAAGCAGCAATTTGCTACCAAAGCGTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAGGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGTCTGGGCAGCGGCCTGGAGTTCGAGGAGATCAAGCTGCTCC
CTGAAGTCACTCAGCAGTTCTATATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTTACCCTGCATGACTTCGAGATCAAGCCGGCTTCGCCCGATAACACCTC
GAAGCTGCGCATGAGCATCTTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZIF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.596

93.237

0.425
  pilO Acinetobacter baumannii D1279779

45.789

91.787

0.42
  comO/pilO Acinetobacter baylyi ADP1

43.617

90.821

0.396