Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   VOI49_RS00775 Genome accession   NZ_CP142018
Coordinates   121485..121865 (+) Length   126 a.a.
NCBI ID   WP_324739576.1    Uniprot ID   -
Organism   Streptococcus dysgalactiae strain lu24     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 116485..126865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VOI49_RS00745 - 118106..118471 (+) 366 WP_003049248.1 DUF1033 family protein -
  VOI49_RS00750 comGA 118552..119493 (+) 942 WP_003049251.1 competence type IV pilus ATPase ComGA Machinery gene
  VOI49_RS00755 comGB 119429..120460 (+) 1032 WP_226313561.1 competence type IV pilus assembly protein ComGB Machinery gene
  VOI49_RS00760 comGC 120462..120788 (+) 327 WP_115246891.1 competence type IV pilus major pilin ComGC Machinery gene
  VOI49_RS00765 comGD 120763..121191 (+) 429 WP_324739066.1 competence type IV pilus minor pilin ComGD Machinery gene
  VOI49_RS00770 comGE 121148..121444 (+) 297 WP_003061797.1 competence type IV pilus minor pilin ComGE Machinery gene
  VOI49_RS00775 comGF 121485..121865 (+) 381 WP_324739576.1 competence type IV pilus minor pilin ComGF Machinery gene
  VOI49_RS00780 comGG 121843..122217 (+) 375 WP_003049269.1 competence type IV pilus minor pilin ComGG -
  VOI49_RS00785 comGH 122280..123233 (+) 954 WP_115246884.1 class I SAM-dependent methyltransferase Machinery gene
  VOI49_RS00790 - 123292..124488 (+) 1197 WP_003049273.1 acetate kinase -
  VOI49_RS00795 - 124798..125004 (+) 207 WP_003058508.1 helix-turn-helix transcriptional regulator -
  VOI49_RS00800 - 125160..125765 (+) 606 WP_226313560.1 CPBP family intramembrane glutamic endopeptidase -
  VOI49_RS00805 - 125783..126220 (+) 438 WP_003049278.1 hypothetical protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 14748.82 Da        Isoelectric Point: 10.2837

>NTDB_id=839150 VOI49_RS00775 WP_324739576.1 121485..121865(+) (comGF) [Streptococcus dysgalactiae strain lu24]
MVISGSLLVYQGLTKTLLSHSRYLTNHDQDHWLLFSQQLREELSGVRFHKVENNKLYIEKGKKKLVLGQFKSHDFRKSAG
NGQGYQPMLFGLSHSHIQAEQSRIRITLHWKSGLERTFYYAFQDQP

Nucleotide


Download         Length: 381 bp        

>NTDB_id=839150 VOI49_RS00775 WP_324739576.1 121485..121865(+) (comGF) [Streptococcus dysgalactiae strain lu24]
CTGGTGATATCAGGTTCTCTTCTAGTGTATCAAGGGTTGACCAAAACACTCCTTAGCCACAGTCGCTACCTTACCAACCA
TGATCAAGACCATTGGCTTTTATTCTCCCAGCAGCTACGAGAAGAGTTAAGTGGAGTGCGATTTCACAAAGTAGAAAACA
ATAAATTATATATTGAAAAAGGCAAGAAAAAACTAGTTTTAGGACAATTTAAAAGCCATGACTTTCGAAAATCGGCAGGC
AATGGACAAGGTTATCAACCCATGCTGTTTGGTCTGTCTCATAGTCATATACAAGCAGAGCAGTCTCGTATTCGTATTAC
CCTTCACTGGAAAAGTGGACTAGAAAGGACCTTCTACTATGCGTTTCAAGACCAACCTTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(20-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Streptococcus mutans UA159

45.968

98.413

0.452
  comGF Streptococcus mutans UA140

45.161

98.413

0.444
  comGF Lactococcus lactis subsp. cremoris KW2

44.355

98.413

0.437
  comGF/cglF Streptococcus pneumoniae Rx1

42.5

95.238

0.405
  comGF/cglF Streptococcus pneumoniae D39

42.5

95.238

0.405
  comGF/cglF Streptococcus pneumoniae R6

42.5

95.238

0.405
  comGF/cglF Streptococcus pneumoniae TIGR4

42.5

95.238

0.405
  comGF/cglF Streptococcus mitis NCTC 12261

42.5

95.238

0.405
  comGF/cglF Streptococcus mitis SK321

41.667

95.238

0.397