Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   VFH86_RS11495 Genome accession   NZ_CP142014
Coordinates   2560169..2561896 (-) Length   575 a.a.
NCBI ID   WP_032478787.1    Uniprot ID   -
Organism   Vibrio cholerae strain LK-18     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2555169..2566896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VFH86_RS11460 (VFH86_11460) - 2555434..2556204 (-) 771 WP_114709990.1 type II secretion system protein -
  VFH86_RS11465 (VFH86_11465) - 2556204..2556770 (-) 567 WP_162808769.1 type II secretion system protein -
  VFH86_RS11470 (VFH86_11470) - 2556760..2557272 (-) 513 WP_050555758.1 type II secretion system protein -
  VFH86_RS11475 (VFH86_11475) - 2557333..2557812 (-) 480 WP_080299312.1 type II secretion system protein -
  VFH86_RS11480 (VFH86_11480) - 2557847..2558401 (-) 555 WP_000738707.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VFH86_RS11485 (VFH86_11485) - 2558482..2558934 (-) 453 WP_095461811.1 MSHA biogenesis protein MshF -
  VFH86_RS11490 (VFH86_11490) - 2558937..2560160 (-) 1224 WP_114720672.1 type II secretion system F family protein -
  VFH86_RS11495 (VFH86_11495) pilB/pilB1 2560169..2561896 (-) 1728 WP_032478787.1 GspE/PulE family protein Machinery gene
  VFH86_RS11500 (VFH86_11500) - 2561886..2563037 (-) 1152 WP_345657594.1 tetratricopeptide repeat protein -
  VFH86_RS11505 (VFH86_11505) - 2563034..2563879 (-) 846 WP_111674013.1 ExeA family protein -
  VFH86_RS11510 (VFH86_11510) mshL 2563884..2565548 (-) 1665 WP_095461815.1 pilus (MSHA type) biogenesis protein MshL -
  VFH86_RS11515 (VFH86_11515) - 2565563..2565880 (-) 318 WP_000252812.1 MSHA biogenesis protein MshK -
  VFH86_RS11520 (VFH86_11520) gspM 2565873..2566523 (-) 651 WP_345657599.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63858.32 Da        Isoelectric Point: 6.0455

>NTDB_id=839078 VFH86_RS11495 WP_032478787.1 2560169..2561896(-) (pilB/pilB1) [Vibrio cholerae strain LK-18]
MPINKLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQQLSLPVIDLSRANVDIDAVP
LLPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLNHLPDYGFEFVIAPEKQLVDGFDRYYRRTKEIVSFAEQLHA
EHKTNDSFDFEITDSDSDEVTVVKLINSLFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLHEVNIASALVLRLKL
MANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPIQHGESVVMRLLNQSAGVRKLEESGIPPHLLLRLRHQLKRPHGMIL
VTGPTGSGKTTTLYGALNELNTSGKKIITAEDPVEYRISRINQVQVNPKINLDFSTLLRTFLRQDPDIILVGEMRDQETV
EIGLRAALTGHLVLSTLHTNDAVDSALRLIDMGAPGYLVASAVRAVVAQRLVRKVCPDCSGHDEVDEARRQWLVTRFPNQ
VAAKFTRGRGCQNCNLTGYRGRIGVFEMLELDQPMMDCLRANDAVAFSKAARSNTDYKPLLASAMELALQGIVSLDEVMS
LGEGDSSGLVEPIYL

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=839078 VFH86_RS11495 WP_032478787.1 2560169..2561896(-) (pilB/pilB1) [Vibrio cholerae strain LK-18]
GTGCCAATTAATAAACTGCGTAAACGGCTTGGTGACTTGCTGGTTGAAGAGGGGATTGTGAGCGAAGCGCAGCTTGAACA
AGCGCTCAATGCCCAGAAAAATACCGGACGCCGCTTAGGGGATACCTTAATTTCGCTTGGCTTTTTAAGCGAAACCCAGT
TGCTGAACTTTTTGGCGCAGCAATTGAGTTTACCTGTGATCGATTTGAGCCGCGCTAATGTTGATATTGATGCGGTTCCT
TTACTGCCTGAAGTTCATGCCCGCCGTTTGCGGGCGCTGGTGATAGGCCGCAGTGGTGATACGCTGCGCATAGCCATGAG
TGATCCTGCGGATCTGTTTGCTCAAGAAGCCTTGCTCAACCACTTGCCTGATTACGGTTTTGAGTTTGTCATCGCTCCTG
AAAAGCAGTTGGTCGATGGCTTTGATCGTTACTATCGCCGCACCAAAGAAATTGTCTCGTTTGCCGAGCAATTGCATGCC
GAACACAAAACCAATGATAGTTTTGATTTTGAGATCACCGATTCAGATAGCGATGAAGTGACGGTTGTTAAACTGATCAA
CTCGCTGTTTGAAGATGCGATTCAAGTGGGTGCCTCGGATATACACATTGAGCCGGATGCCAACGTACTGCGTTTGCGTC
AACGGATCGATGGGGTACTGCATGAAACCTTGCTCCATGAAGTGAATATCGCCTCAGCCTTAGTGCTGCGCTTAAAATTA
ATGGCGAATCTGGATATTTCAGAAAAACGCCTTCCGCAAGATGGACGCTTTAACATCCGTGTAAAAGGACAATCCGTCGA
TATCCGGATGTCAACCATGCCTATCCAGCACGGTGAATCAGTGGTGATGCGTTTGCTCAACCAATCTGCAGGGGTTAGAA
AGCTCGAAGAGTCCGGTATTCCGCCTCATTTATTGCTTCGTTTGCGCCATCAGTTGAAACGCCCTCATGGCATGATTCTG
GTGACTGGGCCTACCGGCTCAGGGAAAACCACCACCTTGTATGGTGCACTCAACGAGCTCAATACGTCCGGCAAAAAAAT
CATTACTGCGGAAGATCCGGTGGAATACCGAATTTCACGGATCAATCAGGTGCAAGTGAACCCGAAAATCAATCTCGATT
TCTCGACGTTACTGCGCACTTTCTTGCGCCAAGACCCAGATATTATTCTGGTCGGTGAGATGCGTGACCAAGAGACGGTC
GAAATTGGCCTGCGCGCCGCCTTAACCGGTCACTTAGTCTTAAGTACTCTGCACACCAATGATGCGGTCGATAGCGCGCT
GCGTTTGATTGATATGGGGGCGCCCGGTTATCTGGTTGCCAGTGCGGTACGTGCCGTGGTTGCGCAGCGTTTAGTACGAA
AAGTCTGTCCGGATTGTAGTGGACATGATGAGGTGGATGAAGCGCGTCGCCAATGGCTTGTGACGCGTTTCCCTAATCAA
GTTGCCGCCAAATTCACTCGTGGACGCGGCTGCCAAAACTGTAACTTAACCGGTTATCGCGGCCGGATCGGTGTATTCGA
AATGCTTGAGCTGGATCAGCCGATGATGGATTGCTTGCGGGCAAATGATGCTGTGGCCTTCTCTAAAGCCGCACGCAGTA
ACACAGATTATAAACCGCTACTGGCTTCAGCGATGGAGTTAGCGCTGCAAGGTATCGTCAGTCTTGATGAAGTGATGTCG
CTCGGTGAAGGTGATTCCTCTGGTTTGGTTGAACCGATTTATTTGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-149)

Type II/IV secretion system protein (T2SSE)

(189-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

35.596

100

0.374
  pilB Vibrio campbellii strain DS40M4

36.796

98.783

0.363
  pilB Vibrio cholerae strain A1552

36.945

97.913

0.362
  pilB Legionella pneumophila strain ERS1305867

39.245

92.174

0.362
  pilF Thermus thermophilus HB27

37.209

97.217

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.477

96.522

0.362