Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   VLJ87_RS02960 Genome accession   NZ_CP141770
Coordinates   643453..644076 (+) Length   207 a.a.
NCBI ID   WP_003116135.1    Uniprot ID   A0A0H2ZIF6
Organism   Pseudomonas aeruginosa strain PA14-GFP     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 638453..649076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VLJ87_RS02945 - 639140..641611 (-) 2472 WP_003141786.1 penicillin-binding protein 1A -
  VLJ87_RS02950 pilM 641796..642860 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  VLJ87_RS02955 pilN 642860..643456 (+) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  VLJ87_RS02960 pilO 643453..644076 (+) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO Machinery gene
  VLJ87_RS02965 comL/pilP 644073..644597 (+) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  VLJ87_RS02970 pilQ 644651..646783 (+) 2133 WP_003141780.1 type 4a pilus secretin PilQ Machinery gene
  VLJ87_RS02975 aroK 646795..647313 (+) 519 WP_003095833.1 shikimate kinase AroK -
  VLJ87_RS02980 aroB 647363..648469 (+) 1107 WP_003107839.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22958.35 Da        Isoelectric Point: 4.9107

>NTDB_id=837698 VLJ87_RS02960 WP_003116135.1 643453..644076(+) (pilO) [Pseudomonas aeruginosa strain PA14-GFP]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLRDLQTQLEQQAAQEESLKQQFATKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPASPDNTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=837698 VLJ87_RS02960 WP_003116135.1 643453..644076(+) (pilO) [Pseudomonas aeruginosa strain PA14-GFP]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTCCGTGACT
TGCAAACACAGCTCGAACAGCAGGCTGCGCAAGAAGAATCGCTCAAGCAGCAATTTGCTACCAAAGCGTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAGGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGTCTGGGCAGCGGCCTGGAGTTCGAGGAGATCAAGCTGCTCC
CTGAAGTCACTCAGCAGTTCTATATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTTACCCTGCATGACTTCGAGATCAAGCCGGCTTCGCCCGATAACACCTC
GAAGCTGCGCATGAGCATCTTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZIF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.596

93.237

0.425
  pilO Acinetobacter baumannii D1279779

45.789

91.787

0.42
  comO/pilO Acinetobacter baylyi ADP1

43.617

90.821

0.396