Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   U8P30_RS12055 Genome accession   NZ_CP141084
Coordinates   2514413..2515651 (-) Length   412 a.a.
NCBI ID   WP_322882296.1    Uniprot ID   -
Organism   Escherichia coli strain KE48     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2509413..2520651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U8P30_RS12035 (U8P30_12035) dam 2510025..2510861 (-) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -
  U8P30_RS12040 (U8P30_12040) damX 2510968..2512254 (-) 1287 WP_000343172.1 cell division protein DamX -
  U8P30_RS12045 (U8P30_12045) aroB 2512346..2513434 (-) 1089 WP_000439850.1 3-dehydroquinate synthase -
  U8P30_RS12050 (U8P30_12050) aroK 2513491..2514012 (-) 522 WP_000818618.1 shikimate kinase AroK -
  U8P30_RS12055 (U8P30_12055) pilQ 2514413..2515651 (-) 1239 WP_322882296.1 DNA uptake porin HofQ Machinery gene
  U8P30_RS12060 (U8P30_12060) hofP 2515563..2515967 (-) 405 WP_001264136.1 DNA utilization protein HofP -
  U8P30_RS12065 (U8P30_12065) hofO 2515957..2516397 (-) 441 WP_001055743.1 DNA utilization protein HofO -
  U8P30_RS12070 (U8P30_12070) hofN 2516381..2516920 (-) 540 WP_001069336.1 DNA utilization protein HofN -
  U8P30_RS12075 (U8P30_12075) hofM 2516920..2517699 (-) 780 WP_001296474.1 DNA utilization protein HofM -
  U8P30_RS12080 (U8P30_12080) mrcA 2517819..2520371 (+) 2553 WP_001298208.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44809.32 Da        Isoelectric Point: 5.9488

>NTDB_id=836071 U8P30_RS12055 WP_322882296.1 2514413..2515651(-) (pilQ) [Escherichia coli strain KE48]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQARAQANLPLENRNITLQYADAGELAKVGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHIGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=836071 U8P30_RS12055 WP_322882296.1 2514413..2515651(-) (pilQ) [Escherichia coli strain KE48]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGGCAATATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGTGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTCTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGCTGGCCGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

38.48

100

0.393
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio cholerae strain A1552

38.015

100

0.381
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.015

100

0.381
  pilQ Pseudomonas aeruginosa PAK

34.633

100

0.367
  comE Glaesserella parasuis strain SC1401

35.476

100

0.362