Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   U8P30_RS11800 Genome accession   NZ_CP141084
Coordinates   2465818..2466495 (+) Length   225 a.a.
NCBI ID   WP_000178174.1    Uniprot ID   A0A0H2VBG7
Organism   Escherichia coli strain KE48     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2460818..2471495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U8P30_RS11765 (U8P30_11765) gspG 2461298..2461735 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  U8P30_RS11770 (U8P30_11770) gspH 2461743..2462252 (+) 510 WP_001076033.1 type II secretion system minor pseudopilin GspH -
  U8P30_RS11775 (U8P30_11775) gspI 2462249..2462626 (+) 378 WP_001041742.1 type II secretion system minor pseudopilin GspI -
  U8P30_RS11780 (U8P30_11780) gspJ 2462619..2463206 (+) 588 WP_000610637.1 type II secretion system minor pseudopilin GspJ -
  U8P30_RS11785 (U8P30_11785) gspK 2463199..2464182 (+) 984 Protein_2305 type II secretion system minor pseudopilin GspK -
  U8P30_RS11790 (U8P30_11790) gspL 2464197..2465360 (+) 1164 WP_001304915.1 type II secretion system protein GspL -
  U8P30_RS11795 (U8P30_11795) gspM 2465357..2465818 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  U8P30_RS11800 (U8P30_11800) pilD 2465818..2466495 (+) 678 WP_000178174.1 prepilin peptidase GspO Machinery gene
  U8P30_RS11805 (U8P30_11805) bfr 2466524..2467000 (-) 477 WP_000675504.1 bacterioferritin -
  U8P30_RS11810 (U8P30_11810) bfd 2467072..2467266 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  U8P30_RS11815 (U8P30_11815) chiA 2467435..2470137 (-) 2703 WP_000773147.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24866.76 Da        Isoelectric Point: 7.6364

>NTDB_id=836069 U8P30_RS11800 WP_000178174.1 2465818..2466495(+) (pilD) [Escherichia coli strain KE48]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTIPTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=836069 U8P30_RS11800 WP_000178174.1 2465818..2466495(+) (pilD) [Escherichia coli strain KE48]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTACCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATACCCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VBG7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387