Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   K9U73_RS27135 Genome accession   NZ_CP140615
Coordinates   5761220..5762092 (+) Length   290 a.a.
NCBI ID   WP_023084740.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 14182     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5756220..5767092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9U73_RS27110 (K9U73_027100) nadC 5756551..5757399 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K9U73_RS27120 (K9U73_027110) pilA 5757603..5758067 (-) 465 WP_031630343.1 pilin Machinery gene
  K9U73_RS27125 (K9U73_027115) pilB 5758298..5759998 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K9U73_RS27130 (K9U73_027120) pilC 5760002..5761219 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  K9U73_RS27135 (K9U73_027125) pilD 5761220..5762092 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  K9U73_RS27140 (K9U73_027130) coaE 5762089..5762700 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  K9U73_RS27145 (K9U73_027135) yacG 5762697..5762897 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  K9U73_RS27150 (K9U73_027140) - 5762934..5763143 (-) 210 WP_003094660.1 hypothetical protein -
  K9U73_RS27155 (K9U73_027145) - 5763249..5763938 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  K9U73_RS27160 (K9U73_027150) - 5763935..5764405 (-) 471 WP_023086643.1 hypothetical protein -
  K9U73_RS27165 (K9U73_027155) - 5764402..5764827 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  K9U73_RS27170 (K9U73_027160) - 5764960..5765589 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  K9U73_RS27175 (K9U73_027165) - 5765586..5766035 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  K9U73_RS27180 (K9U73_027170) - 5766061..5766231 (+) 171 WP_003094672.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31843.88 Da        Isoelectric Point: 7.7068

>NTDB_id=835573 K9U73_RS27135 WP_023084740.1 5761220..5762092(+) (pilD) [Pseudomonas aeruginosa strain 14182]
MTLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=835573 K9U73_RS27135 WP_023084740.1 5761220..5762092(+) (pilD) [Pseudomonas aeruginosa strain 14182]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCTATCCTCCTTGGCCTGCTGGTTGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTACCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCTTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.714

96.552

0.538
  pilD Vibrio campbellii strain DS40M4

53.004

97.586

0.517
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

49.281

95.862

0.472