Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K9U73_RS27120 Genome accession   NZ_CP140615
Coordinates   5757603..5758067 (-) Length   154 a.a.
NCBI ID   WP_031630343.1    Uniprot ID   Q93PL5
Organism   Pseudomonas aeruginosa strain 14182     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5752603..5763067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9U73_RS27100 (K9U73_027090) ampD 5753293..5753859 (-) 567 WP_023084738.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K9U73_RS27105 (K9U73_027095) - 5754010..5756265 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  K9U73_RS27110 (K9U73_027100) nadC 5756551..5757399 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K9U73_RS27120 (K9U73_027110) pilA 5757603..5758067 (-) 465 WP_031630343.1 pilin Machinery gene
  K9U73_RS27125 (K9U73_027115) pilB 5758298..5759998 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K9U73_RS27130 (K9U73_027120) pilC 5760002..5761219 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  K9U73_RS27135 (K9U73_027125) pilD 5761220..5762092 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  K9U73_RS27140 (K9U73_027130) coaE 5762089..5762700 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  K9U73_RS27145 (K9U73_027135) yacG 5762697..5762897 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 15780.91 Da        Isoelectric Point: 6.1571

>NTDB_id=835570 K9U73_RS27120 WP_031630343.1 5757603..5758067(-) (pilA) [Pseudomonas aeruginosa strain 14182]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVSRSQVSRVMAEAGSLKTAVEACLQDGRTAVGTAAGQCDPGATGSSL
LTGASQTSQTLPTNTGVPQVLDPLTTQTTIIATFGNGASAAISGQTLTWTRDVNGGWSCATTVDAKFRPNGCTD

Nucleotide


Download         Length: 465 bp        

>NTDB_id=835570 K9U73_RS27120 WP_031630343.1 5757603..5758067(-) (pilA) [Pseudomonas aeruginosa strain 14182]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGTATTCTGGCTGCAATCGCAAT
TCCCCAATATCAGAACTATGTTTCTCGCTCTCAGGTCTCCAGGGTTATGGCAGAGGCTGGCTCCTTGAAGACTGCAGTTG
AGGCCTGCCTCCAGGATGGTCGTACTGCTGTGGGTACTGCTGCTGGTCAATGCGACCCGGGTGCGACGGGTTCCAGTTTG
TTGACTGGTGCTTCTCAGACTTCTCAAACCCTGCCAACCAATACCGGTGTTCCGCAGGTTCTGGATCCTCTGACTACTCA
AACCACTATCATTGCGACTTTTGGTAACGGTGCATCCGCAGCTATTTCTGGCCAGACTCTGACCTGGACTCGTGATGTTA
ATGGTGGCTGGAGCTGTGCTACTACCGTAGATGCTAAATTCCGTCCTAATGGCTGTACTGACTGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q93PL5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

46.753

100

0.468
  pilA Vibrio cholerae strain A1552

43.59

100

0.442
  pilA Vibrio cholerae C6706

43.59

100

0.442
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.59

100

0.442
  comP Acinetobacter baylyi ADP1

40.12

100

0.435
  pilA Vibrio parahaemolyticus RIMD 2210633

40.881

100

0.422
  pilA Acinetobacter baumannii strain A118

41.83

99.351

0.416
  pilA2 Legionella pneumophila strain ERS1305867

41.558

100

0.416
  pilA2 Legionella pneumophila str. Paris

40.909

100

0.409
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40

100

0.403
  pilE Neisseria gonorrhoeae MS11

33.908

100

0.383
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.411

98.052

0.377