Detailed information NTG0835149

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: QNH25_RS16705
• Genome accession: NZ_CP126087
• Coordinates: 3253184..3254581 (-)
• Length: 465 a.a.
• NCBI ID: WP_283860161.1
• Uniprot ID: -
• Organism: Bacillus safensis strain CEW-AP100
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3248184..3259581

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QNH25_RS16670 (QNH25_16670)	flgL	3248385..3249293 (-)	909	WP_024423617.1	flagellar hook-associated protein FlgL	-
QNH25_RS16675 (QNH25_16675)	flgK	3249303..3250823 (-)	1521	WP_024423618.1	flagellar hook-associated protein FlgK	-
QNH25_RS16680 (QNH25_16680)	-	3250839..3251321 (-)	483	WP_103131678.1	flagellar protein FlgN	-
QNH25_RS16685 (QNH25_16685)	flgM	3251340..3251603 (-)	264	WP_024423620.1	flagellar biosynthesis anti-sigma factor FlgM	-
QNH25_RS16690 (QNH25_16690)	-	3251683..3252102 (-)	420	WP_024423621.1	TIGR03826 family flagellar region protein	-
QNH25_RS16695 (QNH25_16695)	comFC	3252159..3252815 (-)	657	WP_347343004.1	ComF family protein	Machinery gene
QNH25_RS16700 (QNH25_16700)	-	3252842..3253135 (-)	294	WP_024423623.1	competence protein ComFB	-
QNH25_RS16705 (QNH25_16705)	comFA	3253184..3254581 (-)	1398	WP_283860161.1	DEAD/DEAH box helicase	Machinery gene
QNH25_RS16710 (QNH25_16710)	-	3254702..3255544 (-)	843	WP_024423625.1	DegV family protein	-
QNH25_RS16715 (QNH25_16715)	degU	3255759..3256448 (-)	690	WP_008348273.1	two-component system response regulator DegU	Regulator
QNH25_RS16720 (QNH25_16720)	degS	3256513..3257688 (-)	1176	WP_003213166.1	sensor histidine kinase	Regulator
QNH25_RS16725 (QNH25_16725)	-	3257910..3258578 (+)	669	WP_012011434.1	YigZ family protein	-

Sequence

Protein

• Length: 465 a.a.
• Molecular weight: 53280.22 Da
• Isoelectric Point: 9.9068

>NTDB_id=835149 QNH25_RS16705 WP_283860161.1 3253184..3254581(-) (comFA) [Bacillus safensis strain CEW-AP100]
MNMDKAMELMRQLHSRHLLTVETKCPQSNLDWLEEKGLVNRSPAIERKVNGFTCCRCGVSHKRYFAHSPCEVCQKDCVYC
RSCIMMGKVAECGFLYEWTGPQMVETCRAELTWQGELSKGQKRASERMIEAIKNKFDLLVWAVCGAGKTEVLFHGIEYAL
NQGMNVCIATPRTDVVLELEPRLRKAFQGVTITVLYGGSSQRFQITPLVITTTHQLMRYKNAFDVLIVDEVDAFPYSMDE
RLQFAVLKAMRKNRGVRIYLSATPSKKMTREVSRGKLEAIKIPLRFHEQPLPVPTFHWIGHWKKKLKKNQLPPKVMNWMQ
RHIANRRRILLFVPSIATMKKVTKILREQSLNVEGVSADDPDRKQKVQHFRDYVYDVLVTTTILERGVTIPNVQVGVLGS
ESTIFTESALVQISGRVGRHPDYCTGDVFLFHFGLTRSMKQAKKHIAKMNDTAAKEFSEKQCGFN

Nucleotide

• Length: 1398 bp

>NTDB_id=835149 QNH25_RS16705 WP_283860161.1 3253184..3254581(-) (comFA) [Bacillus safensis strain CEW-AP100]
TTGAATATGGACAAAGCAATGGAACTAATGCGGCAGCTGCATTCACGCCATCTGCTCACCGTTGAAACAAAGTGCCCACA
GTCCAATTTAGATTGGCTGGAGGAGAAGGGCTTAGTCAATCGGTCTCCAGCCATTGAGAGGAAAGTAAATGGTTTTACAT
GCTGCCGGTGCGGTGTATCACACAAGCGGTATTTCGCTCATTCTCCATGTGAAGTGTGTCAAAAGGATTGTGTGTACTGT
AGATCATGCATCATGATGGGAAAAGTAGCAGAATGTGGGTTTCTTTATGAATGGACAGGTCCACAAATGGTAGAAACATG
TCGAGCCGAATTAACATGGCAGGGAGAGCTGTCTAAAGGACAAAAAAGAGCGTCAGAAAGAATGATTGAAGCTATTAAAA
ACAAATTTGATCTACTTGTTTGGGCGGTTTGCGGAGCAGGGAAAACGGAAGTACTTTTTCACGGAATCGAATATGCGTTA
AATCAAGGAATGAATGTCTGTATTGCGACACCTAGAACAGATGTAGTGCTTGAACTCGAACCACGGCTTAGAAAAGCATT
TCAAGGGGTGACAATTACCGTACTTTACGGAGGTAGTTCTCAAAGGTTTCAAATAACACCGCTTGTGATCACCACAACCC
ACCAGCTGATGAGATACAAAAATGCTTTTGATGTGCTCATTGTAGATGAAGTCGATGCCTTCCCTTATTCAATGGATGAG
CGTCTCCAATTTGCTGTTCTAAAGGCGATGAGAAAGAACAGAGGGGTTAGGATTTATTTAAGTGCGACACCCTCTAAAAA
AATGACGAGAGAGGTTTCTCGTGGAAAACTGGAAGCGATAAAAATTCCTTTGCGTTTTCACGAGCAACCATTACCCGTAC
CGACCTTTCATTGGATCGGTCATTGGAAAAAGAAATTAAAAAAGAACCAGCTGCCCCCTAAAGTGATGAATTGGATGCAG
AGACATATTGCAAATAGGAGAAGAATATTACTTTTTGTTCCTTCAATTGCTACCATGAAGAAGGTAACGAAGATTCTTCG
AGAACAATCCTTAAATGTGGAGGGAGTATCTGCCGATGATCCGGATAGGAAACAAAAGGTTCAGCACTTTAGAGATTACG
TATACGATGTACTAGTTACAACCACTATTCTAGAAAGAGGCGTAACGATTCCAAATGTTCAAGTGGGGGTACTAGGTTCG
GAATCTACTATTTTTACAGAGAGTGCACTTGTTCAGATATCTGGACGGGTAGGCAGACACCCGGATTATTGTACAGGAGA
TGTTTTCCTTTTTCATTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAGCATATCGCAAAAATGAATGATACGGCTG
CAAAAGAGTTTTCTGAAAAACAGTGTGGGTTCAACTAA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (324-420)

• Type III restriction enzyme, res subunit (ResIII) (116-265)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	52.412	98.065	0.514
comFA/cflA	Streptococcus pneumoniae Rx1	38.497	94.409	0.363
comFA/cflA	Streptococcus pneumoniae D39	38.497	94.409	0.363
comFA/cflA	Streptococcus pneumoniae R6	38.497	94.409	0.363
comFA/cflA	Streptococcus pneumoniae TIGR4	38.269	94.409	0.361