NTDB

Overview

Name	comFC	Type	Machinery gene
Locus tag	QNH25_RS16695	Genome accession	NZ_CP126087
Coordinates	3252159..3252815 (-)	Length	218 a.a.
NCBI ID	WP_347343004.1	Uniprot ID	-
Organism	Bacillus safensis strain CEW-AP100
Function	ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3247159..3257815

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QNH25_RS16660 (QNH25_16660)	fliW	3247297..3247731 (-)	435	WP_024423615.1	flagellar assembly protein FliW	-
QNH25_RS16665 (QNH25_16665)	-	3247760..3248338 (-)	579	WP_024426253.1	DUF6470 family protein	-
QNH25_RS16670 (QNH25_16670)	flgL	3248385..3249293 (-)	909	WP_024423617.1	flagellar hook-associated protein FlgL	-
QNH25_RS16675 (QNH25_16675)	flgK	3249303..3250823 (-)	1521	WP_024423618.1	flagellar hook-associated protein FlgK	-
QNH25_RS16680 (QNH25_16680)	-	3250839..3251321 (-)	483	WP_103131678.1	flagellar protein FlgN	-
QNH25_RS16685 (QNH25_16685)	flgM	3251340..3251603 (-)	264	WP_024423620.1	flagellar biosynthesis anti-sigma factor FlgM	-
QNH25_RS16690 (QNH25_16690)	-	3251683..3252102 (-)	420	WP_024423621.1	TIGR03826 family flagellar region protein	-
QNH25_RS16695 (QNH25_16695)	comFC	3252159..3252815 (-)	657	WP_347343004.1	ComF family protein	Machinery gene
QNH25_RS16700 (QNH25_16700)	-	3252842..3253135 (-)	294	WP_024423623.1	competence protein ComFB	-
QNH25_RS16705 (QNH25_16705)	comFA	3253184..3254581 (-)	1398	WP_283860161.1	DEAD/DEAH box helicase	Machinery gene
QNH25_RS16710 (QNH25_16710)	-	3254702..3255544 (-)	843	WP_024423625.1	DegV family protein	-
QNH25_RS16715 (QNH25_16715)	degU	3255759..3256448 (-)	690	WP_008348273.1	two-component system response regulator DegU	Regulator
QNH25_RS16720 (QNH25_16720)	degS	3256513..3257688 (-)	1176	WP_003213166.1	sensor histidine kinase	Regulator

Sequence

Protein

Download Length: 218 a.a. Molecular weight: 25037.25 Da Isoelectric Point: 10.0127

                                >NTDB_id=835148 QNH25_RS16695 WP_347343004.1 3252159..3252815(-) (comFC) [Bacillus safensis strain CEW-AP100]

MPAFTWKSFLLLNDDRLCVACKNHLVKIERPICPTCGREQSNDQLCQDCNRWIERSDSIKILVKNRSVYTYNELMKDVLA

RFKFRGDTALAELFKKDVQAAFKHSFCIKDPLLIPIPLSHERLKERGFNQSVKIASLLDKPILQPLVKIHQSKQSKKKKN

ERLDQKGLFQIKQTDAIVQRDIILVDDIYTTGATIYDAARILKDAGAKSVSSFTLIRS

Nucleotide

Download Length: 657 bp

                                >NTDB_id=835148 QNH25_RS16695 WP_347343004.1 3252159..3252815(-) (comFC) [Bacillus safensis strain CEW-AP100]

GTGCCTGCTTTTACATGGAAATCATTCCTACTATTAAATGATGATCGCCTTTGTGTAGCATGTAAGAATCACTTAGTAAA

GATTGAGCGTCCTATTTGTCCAACATGCGGAAGGGAGCAGTCAAATGACCAATTGTGCCAGGACTGTAACAGATGGATTG

AACGCTCTGATTCTATTAAAATCCTTGTGAAAAATAGGTCAGTTTATACTTACAACGAGTTGATGAAAGATGTCCTTGCC

CGCTTTAAATTTCGGGGTGATACGGCTTTAGCTGAGCTGTTTAAAAAAGATGTACAAGCAGCATTTAAACATTCCTTCTG

CATAAAAGACCCATTGCTGATACCGATTCCTTTAAGTCATGAAAGACTAAAAGAAAGAGGATTTAATCAGTCTGTGAAAA

TCGCTTCTTTGCTAGATAAGCCAATATTGCAGCCACTCGTGAAAATACATCAAAGTAAGCAGTCTAAAAAAAAGAAAAAT

GAACGCCTAGATCAAAAAGGATTGTTTCAAATAAAGCAAACAGATGCGATTGTTCAAAGGGATATTATATTAGTTGATGA

TATCTATACAACGGGAGCAACCATCTATGATGCGGCAAGAATTTTGAAAGACGCAGGTGCTAAAAGTGTTTCTTCTTTTA

CGTTGATACGTAGTTAA

Domains

Predicted by InterproScan.

Phosphoribosyl transferase domain (Pribosyltran)

(127-216)

Double zinc ribbon domain (DZR_2)

(14-49)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comFC	Bacillus subtilis subsp. subtilis str. 168	50	99.083	0.495
comFC/cflB	Streptococcus pneumoniae Rx1	36.744	98.624	0.362
comFC/cflB	Streptococcus pneumoniae D39	36.744	98.624	0.362
comFC/cflB	Streptococcus pneumoniae R6	36.744	98.624	0.362
comFC/cflB	Streptococcus pneumoniae TIGR4	36.744	98.624	0.362

Detailed information NTG0835148