Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   QNH33_RS17240 Genome accession   NZ_CP126085
Coordinates   3612536..3613612 (-) Length   358 a.a.
NCBI ID   WP_053537315.1    Uniprot ID   -
Organism   Peribacillus frigoritolerans strain CF20     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3607536..3618612
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QNH33_RS17200 (QNH33_17200) - 3608404..3609201 (+) 798 WP_048688306.1 YqhG family protein -
  QNH33_RS17205 (QNH33_17205) - 3609246..3609431 (-) 186 WP_048688303.1 YqzE family protein -
  QNH33_RS17210 (QNH33_17210) comGG 3609547..3609933 (-) 387 WP_053535692.1 competence type IV pilus minor pilin ComGG -
  QNH33_RS17215 (QNH33_17215) comGF 3609926..3610405 (-) 480 WP_084786101.1 competence type IV pilus minor pilin ComGF -
  QNH33_RS17220 (QNH33_17220) - 3610353..3610691 (-) 339 WP_264895127.1 hypothetical protein -
  QNH33_RS17225 (QNH33_17225) comGD 3610678..3611121 (-) 444 WP_283905735.1 competence type IV pilus minor pilin ComGD -
  QNH33_RS17230 (QNH33_17230) comGC 3611129..3611440 (-) 312 WP_034308384.1 competence type IV pilus major pilin ComGC -
  QNH33_RS17235 (QNH33_17235) comGB 3611518..3612552 (-) 1035 WP_264895128.1 competence type IV pilus assembly protein ComGB -
  QNH33_RS17240 (QNH33_17240) comGA 3612536..3613612 (-) 1077 WP_053537315.1 competence type IV pilus ATPase ComGA Machinery gene
  QNH33_RS17245 (QNH33_17245) - 3613716..3614096 (-) 381 WP_095678573.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  QNH33_RS17250 (QNH33_17250) - 3614312..3615013 (+) 702 WP_283905736.1 helix-turn-helix domain-containing protein -
  QNH33_RS17255 (QNH33_17255) - 3615099..3615341 (+) 243 WP_034308393.1 DUF2626 domain-containing protein -
  QNH33_RS17260 (QNH33_17260) - 3615394..3616494 (-) 1101 WP_164855111.1 SAM-dependent methyltransferase -
  QNH33_RS17265 (QNH33_17265) - 3616808..3617434 (-) 627 WP_283905737.1 MBL fold metallo-hydrolase -
  QNH33_RS17270 (QNH33_17270) - 3617668..3617841 (+) 174 WP_034308396.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39828.45 Da        Isoelectric Point: 8.4751

>NTDB_id=835003 QNH33_RS17240 WP_053537315.1 3612536..3613612(-) (comGA) [Peribacillus frigoritolerans strain CF20]
MISIEKTAEKILTRAIVESASDIHIFFRGEGPHIQFRIDNKLVPQETLSFFEAERLIAHLKFLASMDIGEKRRPQSGAIT
INLANQVVGLRLSTLPTAHLESLVIRLIPQQNILPLEQLSLFPNTVQKLIALLKHSHGMLIFTGPTGSGKTTTLYSLLHH
AHEMINRNIITLEDPIENVSEKVLQVQINEKAGITYSVGLKAVLRHDPDVIMVGEVRDAETAKIAVRAALTGHLILTTMH
TRDAQGAISRLLEFGVSLLEVEQTLIGVTAQRLVELRCLPCKGDCALACKMTARNKRASVYELLYGKSLAEVLRIMGDEK
GKATVSYRQLKDEIGKAVAMGYVESQEYERLVYHETKK

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=835003 QNH33_RS17240 WP_053537315.1 3612536..3613612(-) (comGA) [Peribacillus frigoritolerans strain CF20]
TTGATATCGATTGAAAAGACCGCAGAAAAAATACTAACCCGTGCCATCGTGGAATCGGCATCGGATATCCACATTTTTTT
TCGCGGAGAGGGGCCTCACATCCAATTCAGGATAGACAATAAGCTTGTTCCCCAGGAAACATTGTCATTCTTTGAAGCTG
AAAGGCTGATCGCTCATTTGAAGTTCCTTGCCTCGATGGATATAGGGGAGAAAAGGAGGCCCCAGAGTGGTGCCATCACC
ATCAATTTGGCTAACCAGGTGGTCGGACTCCGCCTTTCCACTTTACCCACTGCCCATCTCGAAAGTTTGGTCATCCGCTT
AATACCCCAACAGAATATCCTTCCTCTAGAACAGTTATCCTTATTTCCAAACACCGTTCAAAAATTGATTGCACTCCTGA
AGCATTCCCATGGCATGCTCATATTTACCGGCCCGACCGGCAGTGGGAAAACCACGACACTATATTCCCTGCTTCACCAC
GCCCATGAGATGATCAATCGAAATATCATTACACTTGAAGATCCCATTGAAAATGTATCCGAAAAGGTATTGCAAGTCCA
AATCAATGAAAAGGCGGGCATTACGTATTCAGTCGGCCTAAAAGCTGTTCTGAGGCATGACCCGGACGTGATCATGGTTG
GGGAAGTCAGGGATGCAGAAACTGCCAAAATCGCGGTCCGTGCCGCATTAACCGGTCATCTGATACTAACGACCATGCAT
ACGAGGGACGCCCAAGGCGCCATCTCCAGATTACTGGAATTTGGTGTCAGCTTACTTGAGGTTGAACAGACTTTGATTGG
CGTGACAGCGCAGCGGCTGGTTGAATTGCGATGTCTTCCGTGTAAAGGGGACTGTGCTTTAGCTTGCAAAATGACTGCAA
GGAATAAAAGGGCAAGTGTATATGAATTGCTATATGGAAAAAGTCTGGCTGAAGTTCTCCGGATAATGGGAGATGAAAAA
GGAAAGGCAACGGTCAGCTACCGCCAATTAAAGGATGAAATCGGAAAAGCTGTTGCGATGGGCTATGTGGAATCTCAGGA
ATATGAACGGCTGGTATACCATGAAACCAAAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.022

99.721

0.559
  pilB Haemophilus influenzae 86-028NP

38.218

97.207

0.372
  pilB Haemophilus influenzae Rd KW20

37.069

97.207

0.36