Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   URF95_RS28795 Genome accession   NZ_CP140111
Coordinates   6209254..6209877 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain 19-3158     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6204254..6214877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  URF95_RS28775 (URF95_28775) aroB 6204850..6205956 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  URF95_RS28780 (URF95_28780) aroK 6206006..6206524 (-) 519 WP_003095833.1 shikimate kinase AroK -
  URF95_RS28785 (URF95_28785) pilQ 6206536..6208680 (-) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  URF95_RS28790 (URF95_28790) comL/pilP 6208733..6209257 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  URF95_RS28795 (URF95_28795) pilO 6209254..6209877 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  URF95_RS28800 (URF95_28800) pilN 6209874..6210470 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  URF95_RS28805 (URF95_28805) pilM 6210470..6211534 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  URF95_RS28810 (URF95_28810) - 6211719..6214190 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=833274 URF95_RS28795 WP_003103268.1 6209254..6209877(-) (pilO) [Pseudomonas aeruginosa strain 19-3158]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=833274 URF95_RS28795 WP_003103268.1 6209254..6209877(-) (pilO) [Pseudomonas aeruginosa strain 19-3158]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401