Detailed information NTG0832485

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QMO63_RS02210
• Genome accession: NZ_CP125729
• Coordinates: 490950..492098 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain SHPHC59
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 485950..497098

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QMO63_RS02185 (QMO63_02185)	-	487101..487397 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
QMO63_RS02190 (QMO63_02190)	-	487394..487987 (-)	594	WP_003084544.1	YggT family protein	-
QMO63_RS02195 (QMO63_02195)	proC	487998..488819 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
QMO63_RS02200 (QMO63_02200)	-	488831..489523 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
QMO63_RS02205 (QMO63_02205)	pilT	489738..490772 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
QMO63_RS02210 (QMO63_02210)	pilU	490950..492098 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
QMO63_RS02215 (QMO63_02215)	czcD	492106..493005 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
QMO63_RS02220 (QMO63_02220)	-	493159..493563 (+)	405	WP_003084560.1	NINE protein	-
QMO63_RS02225 (QMO63_02225)	-	493806..495179 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
QMO63_RS02230 (QMO63_02230)	-	495176..496360 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=832485 QMO63_RS02210 WP_003100959.1 490950..492098(+) (pilU) [Pseudomonas aeruginosa strain SHPHC59]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=832485 QMO63_RS02210 WP_003100959.1 490950..492098(+) (pilU) [Pseudomonas aeruginosa strain SHPHC59]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382