Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QLF99_RS04675 Genome accession   NZ_CP125110
Coordinates   1025166..1026296 (+) Length   376 a.a.
NCBI ID   WP_054663718.1    Uniprot ID   A0A0R0E4U6
Organism   Stenotrophomonas acidaminiphila strain SPDF1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1020166..1031296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QLF99_RS04660 (QLF99_04660) - 1020843..1022801 (+) 1959 WP_282884248.1 alpha/beta fold hydrolase -
  QLF99_RS04665 (QLF99_04665) proC 1022890..1023729 (-) 840 WP_054663712.1 pyrroline-5-carboxylate reductase -
  QLF99_RS04670 (QLF99_04670) pilT 1023996..1025033 (+) 1038 WP_104938226.1 type IV pilus twitching motility protein PilT Machinery gene
  QLF99_RS04675 (QLF99_04675) pilU 1025166..1026296 (+) 1131 WP_054663718.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QLF99_RS04680 (QLF99_04680) - 1026975..1027523 (+) 549 WP_282884665.1 DUF3617 family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41809.11 Da        Isoelectric Point: 7.0672

>NTDB_id=830000 QLF99_RS04675 WP_054663718.1 1025166..1026296(+) (pilU) [Stenotrophomonas acidaminiphila strain SPDF1]
MSTIDFTSFLKLMAHQKASDLFITAGMPPSMKVHGRISPITQTPLTPQQSRDMVLNVMTPAQREEFEKTHECNFAIGLSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELNLPPVIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLATLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNLKGVVAQQLVPTPDGKGRRVAMEILLGTPLVQDYIRDGEIHKLKEVMKESVQLGMKTF
DQSLFELYQAGEISYDDALRHADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAGTR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=830000 QLF99_RS04675 WP_054663718.1 1025166..1026296(+) (pilU) [Stenotrophomonas acidaminiphila strain SPDF1]
ATGAGCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCGCCGGCAT
GCCGCCGTCGATGAAGGTGCATGGCAGGATCAGCCCGATCACGCAGACGCCGCTGACCCCGCAGCAGAGCCGCGACATGG
TGCTCAACGTGATGACGCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCCTGTCCGGC
GTGGGCCGCTTCCGCGTCAGCTGTTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACGCGGATCCC
GACGGTGGAGGAGCTGAACCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCGACCGGGCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAAGTGGGCATCGATACCGACAG
CTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTCCGCACCCGCGAAGGCA
TGGACCACGCCATCGCCTTCGCCGAGACCGGTCACCTGGTGCTGGCGACGCTGCATGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCAGCCAGCTGCTGATGGACCTTTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGGTGCCGACGCCCGATGGCAAGGGGCGCCGGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAATCGGTGCAGCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGACGACGCGCTGCGCCATGCCGATTCCCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAGGGCGGCGATGCCAAGACCCTTTCGCAGGGGCTCGATGGCGTGGAGATCGCCG
GCACGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0R0E4U6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.937

97.606

0.673
  pilU Acinetobacter baylyi ADP1

64.426

94.947

0.612
  pilU Vibrio cholerae strain A1552

54.494

94.681

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.137

94.947

0.41
  pilT Acinetobacter nosocomialis M2

39.535

91.489

0.362
  pilT Acinetobacter baumannii D1279779

39.535

91.489

0.362
  pilT Acinetobacter baumannii strain A118

39.535

91.489

0.362