Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   QKA38_RS25085 Genome accession   NZ_CP124669
Coordinates   5381841..5383178 (+) Length   445 a.a.
NCBI ID   WP_003094694.1    Uniprot ID   A0A0H2ZG94
Organism   Pseudomonas aeruginosa strain 2021CK-01494     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5376841..5388178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QKA38_RS25060 (QKA38_25060) pgeF 5376984..5377712 (-) 729 WP_023092931.1 peptidoglycan editing factor PgeF -
  QKA38_RS25065 (QKA38_25065) rluD 5377709..5378671 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  QKA38_RS25070 (QKA38_25070) - 5378817..5379842 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  QKA38_RS25075 (QKA38_25075) - 5379999..5380244 (+) 246 WP_014603563.1 PP0621 family protein -
  QKA38_RS25080 (QKA38_25080) pilS 5380234..5381826 (+) 1593 WP_003145979.1 two-component system sensor histidine kinase PilS Regulator
  QKA38_RS25085 (QKA38_25085) pilR 5381841..5383178 (+) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  QKA38_RS25090 (QKA38_25090) thiO 5383224..5384318 (-) 1095 WP_023098084.1 glycine oxidase ThiO -
  QKA38_RS25095 (QKA38_25095) - 5384453..5384962 (+) 510 WP_023098085.1 Tfp pilus assembly protein FimT/FimU -
  QKA38_RS25100 (QKA38_25100) - 5385068..5385574 (+) 507 WP_023098086.1 Tfp pilus assembly protein FimT/FimU -
  QKA38_RS25105 (QKA38_25105) pilV 5385565..5386122 (+) 558 WP_003110422.1 type 4a pilus minor pilin PilV Machinery gene
  QKA38_RS25110 (QKA38_25110) pilW 5386119..5386943 (+) 825 WP_003119422.1 type 4a pilus minor pilin PilW -
  QKA38_RS25115 (QKA38_25115) pilX 5386940..5387527 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49737.06 Da        Isoelectric Point: 5.8654

>NTDB_id=827597 QKA38_RS25085 WP_003094694.1 5381841..5383178(+) (pilR) [Pseudomonas aeruginosa strain 2021CK-01494]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=827597 QKA38_RS25085 WP_003094694.1 5381841..5383178(+) (pilR) [Pseudomonas aeruginosa strain 2021CK-01494]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGCAGCGGCAAGGAACTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAGCTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAAGCCGCCAGCGGTGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAAAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGATCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCGGCGAGCCTGAGCGAAATCGACAATCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGTTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-115)

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZG94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

100

100

1
  pilR Acinetobacter baumannii strain A118

51.198

100

0.528
  luxO Vibrio cholerae strain A1552

38.17

100

0.384