Detailed information NTG0082713

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: H7674_RS00675
• Genome accession: NZ_AP023388
• Coordinates: 104216..104650 (+)
• Length: 144 a.a.
• NCBI ID: WP_002992738.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NIH35
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 99216..109650

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H7674_RS00645 (SPNIH35_00800)	-	100885..101250 (+)	366	WP_002986560.1	DUF1033 family protein	-
H7674_RS00650 (SPNIH35_00810)	comYA	101343..102281 (+)	939	WP_011284420.1	competence type IV pilus ATPase ComGA	Machinery gene
H7674_RS00655 (SPNIH35_00820)	comYB	102217..103251 (+)	1035	WP_227868694.1	competence type IV pilus assembly protein ComGB	Machinery gene
H7674_RS00660 (SPNIH35_00830)	comYC	103253..103579 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
H7674_RS00665 (SPNIH35_00840)	comGD	103554..103982 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	-
H7674_RS00670 (SPNIH35_00850)	comGE	103939..104223 (+)	285	WP_011284422.1	competence type IV pilus minor pilin ComGE	-
H7674_RS00675 (SPNIH35_00860)	comYF	104216..104650 (+)	435	WP_002992738.1	competence type IV pilus minor pilin ComGF	Machinery gene
H7674_RS00680 (SPNIH35_00870)	comGG	104634..104960 (+)	327	WP_010921801.1	competence type IV pilus minor pilin ComGG	-
H7674_RS00685 (SPNIH35_00880)	comYH	105058..106011 (+)	954	WP_010921802.1	class I SAM-dependent methyltransferase	Machinery gene
H7674_RS00690 (SPNIH35_00890)	-	106070..107266 (+)	1197	WP_011284424.1	acetate kinase	-
H7674_RS00695 (SPNIH35_00900)	-	107453..107761 (+)	309	WP_011284425.1	hypothetical protein	-
H7674_RS00700 (SPNIH35_00910)	proC	107844..108614 (-)	771	WP_011284426.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16645.27 Da
• Isoelectric Point: 10.2526

>NTDB_id=82713 H7674_RS00675 WP_002992738.1 104216..104650(+) (comYF) [Streptococcus pyogenes strain NIH35]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=82713 H7674_RS00675 WP_002992738.1 104216..104650(+) (comYF) [Streptococcus pyogenes strain NIH35]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	46.715	95.139	0.444
comGF/cglF	Streptococcus mitis NCTC 12261	44.776	93.056	0.417
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396