Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   QI003_RS15750 Genome accession   NZ_CP124601
Coordinates   3154398..3155468 (-) Length   356 a.a.
NCBI ID   WP_041521378.1    Uniprot ID   -
Organism   Bacillus stercoris strain BS21     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3149398..3160468
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QI003_RS15705 (QI003_15705) tapA 3149886..3150641 (-) 756 WP_282372662.1 amyloid fiber anchoring/assembly protein TapA -
  QI003_RS15710 (QI003_15710) - 3150913..3151239 (+) 327 WP_014664589.1 YqzG/YhdC family protein -
  QI003_RS15715 (QI003_15715) - 3151280..3151459 (-) 180 WP_014480252.1 YqzE family protein -
  QI003_RS15720 (QI003_15720) comGG 3151531..3151905 (-) 375 WP_071578591.1 competence type IV pilus minor pilin ComGG Machinery gene
  QI003_RS15725 (QI003_15725) comGF 3151906..3152286 (-) 381 WP_014664591.1 competence type IV pilus minor pilin ComGF Machinery gene
  QI003_RS15730 (QI003_15730) comGE 3152312..3152659 (-) 348 WP_014664592.1 competence type IV pilus minor pilin ComGE Machinery gene
  QI003_RS15735 (QI003_15735) comGD 3152643..3153074 (-) 432 WP_014664593.1 competence type IV pilus minor pilin ComGD Machinery gene
  QI003_RS15740 (QI003_15740) comGC 3153064..3153360 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  QI003_RS15745 (QI003_15745) comGB 3153374..3154411 (-) 1038 WP_282372665.1 competence type IV pilus assembly protein ComGB Machinery gene
  QI003_RS15750 (QI003_15750) comGA 3154398..3155468 (-) 1071 WP_041521378.1 competence protein ComGA Machinery gene
  QI003_RS15755 (QI003_15755) - 3155680..3156090 (-) 411 WP_041521379.1 CBS domain-containing protein -
  QI003_RS15760 (QI003_15760) corA 3156153..3157106 (-) 954 WP_282372667.1 magnesium transporter CorA -
  QI003_RS15765 (QI003_15765) - 3157249..3158577 (+) 1329 WP_069150775.1 hemolysin family protein -
  QI003_RS15770 (QI003_15770) rsbRD 3158627..3159463 (-) 837 WP_014664600.1 RsbT co-antagonist protein RsbRD -
  QI003_RS15780 (QI003_15780) mgsR 3159685..3160065 (-) 381 WP_014664601.1 transcriptional regulator MgsR -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40427.63 Da        Isoelectric Point: 9.1812

>NTDB_id=826632 QI003_RS15750 WP_041521378.1 3154398..3155468(-) (comGA) [Bacillus stercoris strain BS21]
MDSIEKISKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRNMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDDDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRAGVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=826632 QI003_RS15750 WP_041521378.1 3154398..3155468(-) (comGA) [Bacillus stercoris strain BS21]
TTGGATTCAATAGAAAAGATAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATCGTGCC
GAGGGAGCGAGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGAACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCTGCAATGGACATAGGGGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTCCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCTCAATACAATATCCCTTCGATTGATAAACTGTCGTTATTTCCAAAGACAGGAGCCACATTACTCTCATTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTGGTTCAATAC
GCAAAAAAACACTTTAATCGAAATATTGTTACATTAGAGGACCCTGTTGAAACTAGGGACGACGACGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAGGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGATGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTGACGAGCCTTCAT
ACGAGAGATGCAAAGGGCGCAATTTACAGGCTGCTTGAATTCGGTATCAATATGAATGAAATTGAACAGACCGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGTCCATTTTGTGAAAACGGATGTTCATCAGTATATTGCCGTCAGTCAC
GAAATACCAGAAGAGCTGGCGTTTATGAGCTTCTTTATGGGAAAAACCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACACTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

98.876

100

0.989
  pilB Glaesserella parasuis strain SC1401

43.408

87.36

0.379
  pilF Thermus thermophilus HB27

37.11

99.157

0.368