Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   QA540_RS06380 Genome accession   NZ_CP124585
Coordinates   1229472..1230443 (-) Length   323 a.a.
NCBI ID   WP_219492120.1    Uniprot ID   -
Organism   Macrococcus psychrotolerans strain NRL/St 13/116     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1224472..1235443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QA540_RS06345 (QA540_06340) gcvT 1225169..1226254 (-) 1086 WP_219494831.1 glycine cleavage system aminomethyltransferase GcvT -
  QA540_RS06350 (QA540_06345) - 1226392..1226898 (-) 507 WP_219491935.1 shikimate kinase -
  QA540_RS06355 (QA540_06350) comGF 1227059..1227511 (-) 453 WP_420496036.1 competence type IV pilus minor pilin ComGF -
  QA540_RS06360 (QA540_06355) - 1227456..1227698 (-) 243 WP_419894979.1 hypothetical protein -
  QA540_RS06365 (QA540_06360) comGD 1227685..1228110 (-) 426 WP_101037039.1 competence type IV pilus minor pilin ComGD -
  QA540_RS06370 (QA540_06365) comGC 1228103..1228426 (-) 324 WP_283722000.1 competence type IV pilus major pilin ComGC Machinery gene
  QA540_RS06375 (QA540_06370) comGB 1228448..1229521 (-) 1074 WP_420496037.1 competence type IV pilus assembly protein ComGB -
  QA540_RS06380 (QA540_06375) comGA 1229472..1230443 (-) 972 WP_219492120.1 competence type IV pilus ATPase ComGA Machinery gene
  QA540_RS06385 (QA540_06380) - 1230655..1230903 (+) 249 WP_041636023.1 DUF2626 domain-containing protein -
  QA540_RS06390 (QA540_06385) - 1230931..1231551 (-) 621 WP_420496038.1 MBL fold metallo-hydrolase -
  QA540_RS06395 (QA540_06390) - 1231641..1231880 (+) 240 WP_219492126.1 DUF2759 domain-containing protein -
  QA540_RS06400 (QA540_06395) - 1231907..1232218 (-) 312 WP_420496039.1 MTH1187 family thiamine-binding protein -
  QA540_RS06405 (QA540_06400) - 1232218..1233186 (-) 969 WP_420496040.1 ROK family glucokinase -
  QA540_RS06410 (QA540_06405) - 1233183..1233374 (-) 192 WP_133445012.1 YqgQ family protein -
  QA540_RS06415 (QA540_06410) - 1233379..1234806 (-) 1428 WP_420496041.1 rhomboid family intramembrane serine protease -
  QA540_RS06420 (QA540_06415) - 1234803..1235357 (-) 555 WP_420496042.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 37050.64 Da        Isoelectric Point: 6.4160

>NTDB_id=826495 QA540_RS06380 WP_219492120.1 1229472..1230443(-) (comGA) [Macrococcus psychrotolerans strain NRL/St 13/116]
MERLFNEIIEQAILQSASDIHFIPGDKSISIKFRIQGDIEDYSDIDDILFRKLLSYIKFTAHLDVSEKNKAQSGIIHFNL
NNLRYNIRASTLPRSLGDEACVLRIIRQSFIDEYQTDDQILFNQMKKSSGIIIISGPTGSGKSTLMYQLVHFAKNTLKRQ
VISIEDPVEQHIDGIIQVNVNEKAEITYQTAIKAILRCDPDIIMLGEIRDSVVAQQVINAGLSGHLVLTTLHANDCIGAL
FRLKEMGINAVDLYQSINLIINQRLVKRIDGKERVLAYEFLTKKDIEKYLKDKHINYRTLADIFKEMYETNQISQHEFEK
FDL

Nucleotide


Download         Length: 972 bp        

>NTDB_id=826495 QA540_RS06380 WP_219492120.1 1229472..1230443(-) (comGA) [Macrococcus psychrotolerans strain NRL/St 13/116]
ATGGAGAGATTATTCAATGAAATAATAGAGCAGGCAATTTTACAAAGTGCATCAGATATACACTTCATTCCTGGTGATAA
AAGCATATCTATTAAATTTAGGATACAAGGTGATATTGAAGACTATAGCGACATCGACGATATATTATTTAGAAAACTAC
TTTCATATATTAAATTTACAGCACATCTTGATGTATCAGAAAAGAATAAGGCCCAGAGTGGCATAATACATTTTAACCTG
AATAACTTACGATACAATATTCGCGCATCTACTTTACCTCGGTCATTAGGTGATGAAGCATGTGTATTGAGGATTATCAG
ACAAAGTTTTATAGATGAATATCAGACAGATGATCAAATATTGTTCAATCAAATGAAAAAATCAAGCGGTATAATTATCA
TTAGTGGACCAACTGGAAGTGGTAAAAGTACATTAATGTATCAACTTGTACATTTTGCGAAGAATACATTGAAACGACAA
GTAATTTCAATAGAAGATCCTGTAGAACAGCATATTGACGGTATCATACAAGTTAATGTTAACGAAAAAGCAGAGATAAC
GTATCAAACCGCGATAAAGGCAATCCTTAGATGTGATCCAGATATCATTATGCTAGGTGAAATAAGAGATTCAGTCGTAG
CCCAGCAAGTTATCAATGCAGGGTTAAGCGGGCATTTAGTTTTAACGACATTACATGCGAATGATTGCATCGGCGCATTA
TTTCGACTTAAAGAAATGGGAATTAATGCTGTTGATCTATATCAAAGTATTAATTTAATTATCAATCAAAGACTTGTAAA
AAGAATAGATGGAAAGGAGCGTGTTTTAGCCTATGAATTTTTGACAAAAAAGGATATTGAAAAATATTTAAAGGATAAAC
ATATAAACTATAGAACGCTGGCTGACATATTTAAGGAGATGTATGAAACAAATCAGATTTCACAACATGAATTTGAAAAA
TTTGATCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

49.695

100

0.505
  comGA Staphylococcus aureus N315

49.695

100

0.505
  comGA Bacillus subtilis subsp. subtilis str. 168

34.483

100

0.372