Detailed information NTG0824892

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SE147_RS10260
• Genome accession: NZ_CP138342
• Coordinates: 1955852..1957078 (+)
• Length: 408 a.a.
• NCBI ID: WP_003692100.1
• Uniprot ID: Q5F5L4
• Organism: Neisseria gonorrhoeae strain TH1713
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1950852..1962078

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SE147_RS10230 (SE147_10240)	-	1951882..1952013 (-)	132	WP_256346261.1	hypothetical protein	-
SE147_RS10235 (SE147_10245)	dksA	1952028..1952444 (-)	417	WP_003692108.1	RNA polymerase-binding protein DksA	-
SE147_RS10240 (SE147_10250)	proC	1952592..1953383 (-)	792	WP_003696395.1	pyrroline-5-carboxylate reductase	-
SE147_RS10245 (SE147_10255)	-	1953432..1953839 (-)	408	WP_003690202.1	hypothetical protein	-
SE147_RS10250 (SE147_10260)	-	1953839..1954525 (-)	687	WP_003690201.1	YggS family pyridoxal phosphate-dependent enzyme	-
SE147_RS10255 (SE147_10265)	pilT	1954645..1955688 (+)	1044	WP_003690200.1	type IV pilus twitching motility protein PilT	Machinery gene
SE147_RS10260 (SE147_10270)	pilU	1955852..1957078 (+)	1227	WP_003692100.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SE147_RS10265 (SE147_10275)	yccS	1957195..1959345 (+)	2151	WP_003699137.1	YccS family putative transporter	-
SE147_RS10270 (SE147_10280)	-	1960230..1960514 (-)	285	WP_003692093.1	GIY-YIG nuclease family protein	-
SE147_RS10275 (SE147_10285)	-	1960884..1961168 (-)	285	WP_003701816.1	GIY-YIG nuclease family protein	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45698.33 Da
• Isoelectric Point: 7.2909

>NTDB_id=824892 SE147_RS10260 WP_003692100.1 1955852..1957078(+) (pilU) [Neisseria gonorrhoeae strain TH1713]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGTHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=824892 SE147_RS10260 WP_003692100.1 1955852..1957078(+) (pilU) [Neisseria gonorrhoeae strain TH1713]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCACACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACTACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCGGGAATTGGGCTTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGACCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: Q5F5L4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.875	88.48	0.397
pilU	Pseudomonas stutzeri DSM 10701	45.225	87.255	0.395
pilU	Vibrio cholerae strain A1552	47.024	82.353	0.387