Detailed information NTG0823490

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: QEP20_RS12865
• Genome accession: NZ_CP123977
• Coordinates: 2496078..2496695 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis subsp. subtilis strain WH60A
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2491078..2501695

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QEP20_RS12835 (QEP20_12835)	rpsT	2491387..2491653 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
QEP20_RS12840 (QEP20_12840)	holA	2491668..2492711 (-)	1044	WP_015714286.1	DNA polymerase III subunit delta	-
QEP20_RS12845 (QEP20_12845)	-	2492751..2492900 (-)	150	WP_003229985.1	hypothetical protein	-
QEP20_RS12850 (QEP20_12850)	yqzM	2492941..2493075 (+)	135	WP_003229983.1	YqzM family protein	-
QEP20_RS12855 (QEP20_12855)	comEC	2493115..2495445 (-)	2331	WP_080477815.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
QEP20_RS12860 (QEP20_12860)	comEB	2495442..2496011 (-)	570	WP_015714288.1	ComE operon protein 2	-
QEP20_RS12865 (QEP20_12865)	comEA	2496078..2496695 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
QEP20_RS12870 (QEP20_12870)	comER	2496779..2497600 (+)	822	WP_004398597.1	late competence protein ComER	-
QEP20_RS12875 (QEP20_12875)	yqeM	2497666..2498409 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
QEP20_RS12880 (QEP20_12880)	rsfS	2498406..2498762 (-)	357	WP_003229971.1	ribosome silencing factor	-
QEP20_RS12885 (QEP20_12885)	yqeK	2498780..2499340 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
QEP20_RS12890 (QEP20_12890)	nadD	2499330..2499899 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
QEP20_RS12895 (QEP20_12895)	yhbY	2499911..2500201 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
QEP20_RS12900 (QEP20_12900)	aroE	2500195..2501037 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=823490 QEP20_RS12865 WP_004398514.1 2496078..2496695(-) (comEA) [Bacillus subtilis subsp. subtilis strain WH60A]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=823490 QEP20_RS12865 WP_004398514.1 2496078..2496695(-) (comEA) [Bacillus subtilis subsp. subtilis strain WH60A]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39