Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R5Y32_RS03400 Genome accession   NZ_CP137529
Coordinates   691525..692751 (-) Length   408 a.a.
NCBI ID   WP_002218483.1    Uniprot ID   Q9K1N4
Organism   Neisseria meningitidis strain EXNM666     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 686525..697751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5Y32_RS03395 yccS 689089..691239 (-) 2151 WP_154074399.1 YccS family putative transporter -
  R5Y32_RS03400 pilU 691525..692751 (-) 1227 WP_002218483.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R5Y32_RS03405 pilT 692914..693957 (-) 1044 WP_002226887.1 type IV pilus twitching motility protein PilT Machinery gene
  R5Y32_RS03410 - 694077..694763 (+) 687 WP_002239404.1 YggS family pyridoxal phosphate-dependent enzyme -
  R5Y32_RS03415 - 694763..695170 (+) 408 WP_002221801.1 hypothetical protein -
  R5Y32_RS03420 proC 695201..696010 (+) 810 WP_228376091.1 pyrroline-5-carboxylate reductase -
  R5Y32_RS03425 dksA 696157..696573 (+) 417 WP_002231430.1 RNA polymerase-binding protein DksA -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45674.28 Da        Isoelectric Point: 6.9640

>NTDB_id=822033 R5Y32_RS03400 WP_002218483.1 691525..692751(-) (pilU) [Neisseria meningitidis strain EXNM666]
MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=822033 R5Y32_RS03400 WP_002218483.1 691525..692751(-) (pilU) [Neisseria meningitidis strain EXNM666]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAATGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCGCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAGTCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACCACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCAGGAATTGGGCCTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCAGCCATATCGTTACCATCGAAGACCCGATTGAATTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAGTCCCCCGACGTGGTCTGCATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATCGACATCGCCC
TCAACCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAACAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGATCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(49-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9K1N4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

44.598

88.48

0.395
  pilU Pseudomonas stutzeri DSM 10701

44.663

87.255

0.39
  pilU Vibrio cholerae strain A1552

46.429

82.353

0.382