Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   R5028_RS25035 Genome accession   NZ_CP137486
Coordinates   5331920..5332543 (+) Length   207 a.a.
NCBI ID   WP_003116135.1    Uniprot ID   A0A0H2ZIF6
Organism   Pseudomonas aeruginosa strain HPA2660     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5326920..5337543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5028_RS25020 (R5028_25020) - 5327607..5330078 (-) 2472 WP_003095841.1 penicillin-binding protein 1A -
  R5028_RS25025 (R5028_25025) pilM 5330263..5331327 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  R5028_RS25030 (R5028_25030) pilN 5331327..5331923 (+) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  R5028_RS25035 (R5028_25035) pilO 5331920..5332543 (+) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO Machinery gene
  R5028_RS25040 (R5028_25040) comL/pilP 5332540..5333064 (+) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  R5028_RS25045 (R5028_25045) pilQ 5333118..5335250 (+) 2133 WP_003116137.1 type 4a pilus secretin PilQ Machinery gene
  R5028_RS25050 (R5028_25050) aroK 5335262..5335780 (+) 519 WP_003095833.1 shikimate kinase AroK -
  R5028_RS25055 (R5028_25055) aroB 5335830..5336936 (+) 1107 WP_003162482.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22958.35 Da        Isoelectric Point: 4.9107

>NTDB_id=820602 R5028_RS25035 WP_003116135.1 5331920..5332543(+) (pilO) [Pseudomonas aeruginosa strain HPA2660]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLRDLQTQLEQQAAQEESLKQQFATKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPASPDNTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=820602 R5028_RS25035 WP_003116135.1 5331920..5332543(+) (pilO) [Pseudomonas aeruginosa strain HPA2660]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTCCGTGACT
TGCAAACACAGCTCGAACAGCAGGCTGCGCAAGAAGAATCGCTCAAGCAGCAATTTGCTACCAAAGCGTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAAGAGATGGAGGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACTGGTCTGGGCAGCGGCCTGGAGTTCGAGGAGATCAAGCTGCTCC
CTGAAGTCACTCAGCAGTTCTATATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTTACCCTGCATGACTTCGAGATCAAGCCGGCTTCGCCCGATAACACCTC
GAAGCTGCGCATGAGCATCCTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZIF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.596

93.237

0.425
  pilO Acinetobacter baumannii D1279779

45.789

91.787

0.42
  comO/pilO Acinetobacter baylyi ADP1

43.617

90.821

0.396