Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   R4540_RS00100 Genome accession   NZ_CP137135
Coordinates   27506..29233 (+) Length   575 a.a.
NCBI ID   WP_001122154.1    Uniprot ID   Q9KUV7
Organism   Vibrio cholerae V52     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 22506..34233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4540_RS00075 (R4540_00075) gspM 22864..23514 (+) 651 WP_000835144.1 type II secretion system protein GspM -
  R4540_RS00080 (R4540_00080) - 23507..23824 (+) 318 WP_000252812.1 MSHA biogenesis protein MshK -
  R4540_RS00085 (R4540_00085) mshL 23839..25518 (+) 1680 WP_001227124.1 pilus (MSHA type) biogenesis protein MshL -
  R4540_RS00090 (R4540_00090) - 25523..26368 (+) 846 WP_000281360.1 ExeA family protein -
  R4540_RS00095 (R4540_00095) - 26365..27516 (+) 1152 WP_000117841.1 lipopolysaccharide assembly protein LapB -
  R4540_RS00100 (R4540_00100) pilB/pilB1 27506..29233 (+) 1728 WP_001122154.1 GspE/PulE family protein Machinery gene
  R4540_RS00105 (R4540_00105) - 29242..30465 (+) 1224 WP_000190444.1 type II secretion system F family protein -
  R4540_RS00110 (R4540_00110) - 30468..30920 (+) 453 WP_000689647.1 MSHA biogenesis protein MshF -
  R4540_RS00115 (R4540_00115) - 31001..31555 (+) 555 WP_000738708.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  R4540_RS00120 (R4540_00120) - 31590..32117 (+) 528 WP_000828585.1 type II secretion system protein -
  R4540_RS00125 (R4540_00125) - 32154..32642 (+) 489 WP_000691266.1 Tfp pilus assembly protein FimT/FimU -
  R4540_RS00130 (R4540_00130) - 32632..33201 (+) 570 WP_001144509.1 type II secretion system protein -
  R4540_RS00135 (R4540_00135) - 33201..33971 (+) 771 WP_000086312.1 PilW family protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63844.29 Da        Isoelectric Point: 6.0455

>NTDB_id=820293 R4540_RS00100 WP_001122154.1 27506..29233(+) (pilB/pilB1) [Vibrio cholerae V52]
MPINKLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQQLSLPVIDLSRAHVDIDAVP
LLPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLNQLPDYGFEFVIAPEKQLVDGFDRYYRRTKEIVSFAEQLHA
EHKTNDSFDFEITDSDSDEVTVVKLINSLFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLHEVNIASALVLRLKL
MANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPIQHGESVVMRLLNQSAGVRKLEESGIPPHLLLRLRHQLKRPHGMIL
VTGPTGSGKTTTLYGALNELNTSGKKIITAEDPVEYRISRINQVQVNPKINLDFSTLLRTFLRQDPDIILVGEMRDQETV
EIGLRAALTGHLVLSTLHTNDAVDSALRLIDMGAPGYLVASAVRAVVAQRLVRKVCPDCSGHDEVDEARRQWLVTRFPNQ
AAAKFTRGRGCQNCNLTGYRGRIGVFEMLELDQPMMDCLRANDAVAFSKAARSNTDYKPLLASAMELALQGIVSLDEVMS
LGEGDSSGLVEPIYL

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=820293 R4540_RS00100 WP_001122154.1 27506..29233(+) (pilB/pilB1) [Vibrio cholerae V52]
GTGCCAATTAATAAACTGCGTAAACGGCTTGGTGACTTGCTGGTTGAAGAGGGGATTGTGAGCGAAGCGCAGCTTGAACA
AGCGCTCAATGCCCAGAAAAATACCGGACGCCGCTTAGGGGATACCTTAATTTCGCTTGGCTTTTTAAGCGAAACCCAGT
TGCTGAACTTTTTGGCGCAGCAATTGAGTTTGCCAGTGATCGATCTGAGCCGTGCCCATGTCGATATTGATGCTGTTCCT
TTACTGCCTGAAGTTCATGCCCGCCGTTTGCGTGCGCTGGTGATAGGCCGCAGTGGTGATACGCTGCGCATAGCCATGAG
TGATCCTGCGGATTTGTTTGCTCAAGAAGCCTTGCTCAACCAGTTGCCTGATTATGGTTTTGAGTTTGTCATCGCCCCTG
AGAAGCAGTTGGTCGATGGCTTTGATCGTTACTATCGCCGCACCAAAGAAATTGTCTCGTTTGCCGAGCAATTGCATGCC
GAACACAAAACCAATGATAGTTTTGATTTTGAGATCACCGATTCAGATAGCGATGAAGTGACGGTTGTTAAACTGATCAA
CTCGCTGTTTGAAGATGCGATTCAAGTGGGAGCCTCGGATATACATATTGAGCCGGATGCCAACGTACTGCGTTTGCGCC
AACGGATCGATGGGGTATTGCATGAAACCTTGCTCCATGAAGTGAATATCGCCTCAGCCTTAGTGCTGCGCTTAAAATTA
ATGGCGAATCTGGATATTTCAGAAAAACGCCTTCCGCAAGATGGCCGCTTTAACATCCGTGTAAAAGGACAATCCGTCGA
TATCCGGATGTCAACCATGCCTATCCAGCACGGTGAATCGGTGGTGATGCGTTTGCTCAACCAATCTGCAGGGGTTAGAA
AGCTCGAAGAGTCAGGTATTCCGCCTCATTTATTGCTGCGTTTGCGCCATCAGTTGAAACGCCCTCATGGCATGATTCTG
GTGACTGGGCCTACCGGCTCGGGGAAAACCACCACCTTGTATGGCGCACTCAACGAGCTCAATACGTCCGGCAAAAAAAT
CATTACTGCGGAAGATCCGGTGGAATACCGGATTTCACGGATCAATCAGGTGCAAGTGAACCCGAAAATCAATCTCGATT
TCTCGACGTTACTGCGCACTTTCTTGCGCCAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCAAGAGACGGTC
GAAATTGGCCTGCGCGCTGCATTAACCGGTCACTTAGTCTTAAGTACTCTGCACACCAATGATGCCGTCGATAGCGCGCT
GCGTTTGATTGATATGGGGGCGCCCGGTTATCTGGTTGCCAGTGCGGTACGTGCCGTGGTTGCGCAGCGTTTAGTGCGAA
AAGTCTGTCCAGATTGTAGTGGACATGATGAGGTGGATGAAGCGCGTCGCCAATGGCTTGTGACGCGTTTCCCTAATCAA
GCTGCCGCCAAATTCACTCGTGGACGCGGCTGCCAGAACTGTAACTTAACCGGTTATCGCGGCCGGATCGGTGTATTTGA
AATGCTTGAGCTGGATCAGCCGATGATGGATTGCTTGCGGGCAAATGATGCTGTGGCCTTCTCTAAAGCCGCACGCAGTA
ACACAGATTATAAACCGCTACTGGCTTCGGCGATGGAGTTAGCGCTACAAGGCATCGTCAGTCTTGATGAAGTGATGTCG
CTGGGTGAAGGTGATTCCTCTGGTTTGGTTGAACCGATTTATCTGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-149)

Type II/IV secretion system protein (T2SSE)

(189-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5HTL

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

35.596

100

0.374
  pilB Vibrio campbellii strain DS40M4

36.796

98.783

0.363
  pilB Vibrio cholerae strain A1552

36.945

97.913

0.362
  pilF Thermus thermophilus HB27

37.209

97.217

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.477

96.522

0.362
  pilB Legionella pneumophila strain ERS1305867

39.057

92.174

0.36