Detailed information NTG0820052

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QEP68_RS14885
• Genome accession: NZ_CP123215
• Coordinates: 3351252..3352421 (-)
• Length: 389 a.a.
• NCBI ID: WP_005732021.1
• Uniprot ID: A0A108V2M6
• Organism: Pseudomonas amygdali pv. aesculi strain 2203
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3346252..3357421

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QEP68_RS14870	yejB	3347536..3348600 (+)	1065	WP_010215968.1	microcin C ABC transporter permease YejB	-
QEP68_RS14875	-	3348600..3349622 (+)	1023	WP_005732025.1	ABC transporter permease	-
QEP68_RS14880	-	3349624..3351201 (+)	1578	WP_010206223.1	ABC transporter ATP-binding protein	-
QEP68_RS14885	pilU	3351252..3352421 (-)	1170	WP_005732021.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QEP68_RS14890	-	3352424..3352546 (-)	123	WP_280124737.1	hypothetical protein	-
QEP68_RS14895	-	3352545..3352820 (+)	276	WP_005732020.1	peptidylprolyl isomerase	-
QEP68_RS14900	pgm	3352895..3354541 (-)	1647	WP_005732019.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
QEP68_RS14905	-	3354657..3356984 (-)	2328	WP_010206225.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42650.81 Da
• Isoelectric Point: 5.8676

>NTDB_id=820052 QEP68_RS14885 WP_005732021.1 3351252..3352421(-) (pilU) [Pseudomonas amygdali pv. aesculi strain 2203]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVIAQGLMDEEQKLEFLRELEMNLAVSLPGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGVASLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=820052 QEP68_RS14885 WP_005732021.1 3351252..3352421(-) (pilU) [Pseudomonas amygdali pv. aesculi strain 2203]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTATCTTTCCACCGGAGCGCCTCCGTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGTTGGGCAGTGAAACCCTCAAACCGGGCGAGGTGGCGGTGATTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAATTCCTGCGCGAACTGGAGATGAACCTTGCTGTTTCGCTGCCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGATATTCCGCGTTTCGA
GGACCTGTTCCTGCCGCCCGTACTGCTCGATGTGATCATGGAAAAGCACGGGCTGGTGTTGTTCGTCGGTGCCACCGGTT
CGGGCAAATCGACCTCGCTGGCGGCGCTGATCGATTACCGCAATCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTCATCCATCGGCACAAGAAGTCCATCGTCAACCAGCGCGAGGTGGGGGTCGATACCCGCAGTTTCCGCGC
GGCGCTGAAGAACACGTTGCGTCAGGCTCCGGATGTGATTCTGATCGGCGAAATTCGTGACCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCATGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGTCGTGCGCAGTTGCTGCATGACCTGGGCAATAACCTCAAGGCGTTCGTGTCCCAGCG
GCTGGTTAGAACCCCGGACGGCAAACGTCGCGCAGCGGTGGAAGTGATGATGGGCACGCCGACCATTCGCGACCTGATTC
AGCGCAATGAACTGACCGAGCTCAAGGGCATCATGGAGAAATCCGGCAGCCTCGGTATGCAGACCTTCGACACGGCGTTG
TTCAATCTGGCCGTCGAGGGGGCGATCAGCGAGGAGGAGGCGCTGAAGAATGCCGACTCGCAGAACAACGTTCGTCTGCG
CCTCAAACTGCACAGTGAAGGCGGAGTCGCCAGCCTGACGACGCCGCCCCCGGCCCCGACCGGCAGCAGCACGGCAAGTA
CCGCCGAGTGGGGGCTGGTCGATGACGACGAGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-270)

3D structure

• AlphaFold DB: A0A108V2M6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.072	99.486	0.558
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	51.714	89.974	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.687	98.458	0.391
pilT	Pseudomonas aeruginosa PAK	41.399	88.175	0.365