Detailed information NTG0820043

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: QEP68_RS05455
• Genome accession: NZ_CP123215
• Coordinates: 1216082..1216954 (-)
• Length: 290 a.a.
• NCBI ID: WP_010216946.1
• Uniprot ID: A0A0Q0CUR9
• Organism: Pseudomonas amygdali pv. aesculi strain 2203
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1211082..1221954

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QEP68_RS05425	-	1211990..1213288 (-)	1299	WP_005732325.1	NAD(P)/FAD-dependent oxidoreductase	-
QEP68_RS30190	-	1213385..1213621 (-)	237	WP_327203937.1	DUF3094 family protein	-
QEP68_RS05430	-	1213506..1214039 (-)	534	WP_005732326.1	MOSC domain-containing protein	-
QEP68_RS05435	-	1214036..1214665 (-)	630	WP_003430735.1	DUF1780 domain-containing protein	-
QEP68_RS05440	-	1214807..1215058 (+)	252	Protein_1071	hypothetical protein	-
QEP68_RS05445	yacG	1215256..1215465 (-)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
QEP68_RS05450	coaE	1215462..1216085 (-)	624	WP_005732328.1	dephospho-CoA kinase	-
QEP68_RS05455	pilD	1216082..1216954 (-)	873	WP_010216946.1	A24 family peptidase	Machinery gene
QEP68_RS05460	pilC	1216956..1218173 (-)	1218	WP_005732330.1	type II secretion system F family protein	Machinery gene
QEP68_RS05465	pilB	1218176..1219870 (-)	1695	WP_010216952.1	type IV-A pilus assembly ATPase PilB	Machinery gene
QEP68_RS05470	-	1220098..1220526 (+)	429	WP_005732335.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32108.25 Da
• Isoelectric Point: 8.5797

>NTDB_id=820043 QEP68_RS05455 WP_010216946.1 1216082..1216954(-) (pilD) [Pseudomonas amygdali pv. aesculi strain 2203]
MPLLDFLASSPLAFVTTCCILGLIIGSFLNVVVYRLPKMMERDWKAQSREMLGLPAEPDQPAFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQTAAMLVLSWGLLTMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=820043 QEP68_RS05455 WP_010216946.1 1216082..1216954(-) (pilD) [Pseudomonas amygdali pv. aesculi strain 2203]
ATGCCCCTCCTCGATTTCCTGGCCAGCTCCCCTCTGGCCTTCGTCACCACCTGCTGCATCCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTAGTCGTCTACCGCCTCCCCAAAATGATGGAGCGCGACTGGAAAGCCCAATCCCGCGAAATGCTCGGCC
TGCCCGCGGAACCCGATCAGCCCGCCTTCAACCTGAACCGCCCTCGTTCCAGCTGCCCGCACTGCGCGCACAAAATCCGC
CCGTGGGAAAACCTGCCGGTCATCAGCTATCTGCTGCTGCGCGGTAAATGCTCACAATGCAAAGCCCCCATCAGCAAACG
CTACCCATTGGTAGAGCTGACCTGCGCAGTGTTGTCGGCCTACGTCGCCTGGCATTTCGGTTTCGGCTGGCAAACGGCCG
CGATGCTGGTGTTGAGCTGGGGCCTGCTGACGATGAGTCTGATCGACGCCGATCACCAGTTACTCCCTGACTCTCTGGTA
CTGCCACTGCTGTGGCTAGGCCTCATCGTCAACGCTTTCGGGCTGTTCACCTCGCTGAACGATGCGCTTTGGGGTGCGGT
GGCCGGTTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTTGGCGCATGGGGCGGCTGGCAGATTCTGCCGTTGACCATCCTGCTGTCATCGCTGGTGGGC
GCGGTGCTTGGGGTGATAATGATGCGCGTGCGCAGGGTTGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

3D structure

• AlphaFold DB: A0A0Q0CUR9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.986	95.172	0.514
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter baumannii D1279779	51.056	97.931	0.5
pilD	Acinetobacter nosocomialis M2	50	97.931	0.49
pilD	Neisseria gonorrhoeae MS11	51.493	92.414	0.476