Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   QAO20_RS00440 Genome accession   NZ_CP123106
Coordinates   77697..78194 (+) Length   165 a.a.
NCBI ID   WP_001798585.1    Uniprot ID   -
Organism   Staphylococcus aureus strain IT-MSSA50     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 72697..83194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QAO20_RS00400 (QAO20_00400) - 72741..73727 (+) 987 WP_000161315.1 ROK family glucokinase -
  QAO20_RS00405 (QAO20_00405) - 73727..74056 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  QAO20_RS00410 (QAO20_00410) - 74053..74676 (+) 624 WP_001223003.1 MBL fold metallo-hydrolase -
  QAO20_RS00415 (QAO20_00415) comGA 74728..75702 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  QAO20_RS00420 (QAO20_00420) comGB 75674..76744 (+) 1071 WP_000776415.1 competence type IV pilus assembly protein ComGB Machinery gene
  QAO20_RS00425 (QAO20_00425) comGC 76758..77069 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  QAO20_RS00430 (QAO20_00430) comGD 77047..77493 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  QAO20_RS00435 (QAO20_00435) comGE 77480..77779 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  QAO20_RS00440 (QAO20_00440) comGF 77697..78194 (+) 498 WP_001798585.1 competence type IV pilus minor pilin ComGF Machinery gene
  QAO20_RS00445 (QAO20_00445) - 78291..78437 (+) 147 WP_001789879.1 hypothetical protein -
  QAO20_RS00450 (QAO20_00450) - 78427..78951 (+) 525 WP_001015120.1 shikimate kinase -
  QAO20_RS00455 (QAO20_00455) gcvT 79110..80201 (+) 1092 WP_000093343.1 glycine cleavage system aminomethyltransferase GcvT -
  QAO20_RS00460 (QAO20_00460) gcvPA 80221..81567 (+) 1347 WP_000019699.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  QAO20_RS00465 (QAO20_00465) gcvPB 81560..83032 (+) 1473 WP_000202177.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19242.06 Da        Isoelectric Point: 10.2192

>NTDB_id=818802 QAO20_RS00440 WP_001798585.1 77697..78194(+) (comGF) [Staphylococcus aureus strain IT-MSSA50]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLIAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNMQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=818802 QAO20_RS00440 WP_001798585.1 77697..78194(+) (comGF) [Staphylococcus aureus strain IT-MSSA50]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAATAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTTATTGAAAGTAGGGAATTAACAACTGTAGATTTTGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATATGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.364

100

0.964