Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   QAO20_RS00415 Genome accession   NZ_CP123106
Coordinates   74728..75702 (+) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain IT-MSSA50     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 69728..80702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QAO20_RS00380 (QAO20_00380) rpmG 70188..70337 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  QAO20_RS00385 (QAO20_00385) - 70546..71085 (+) 540 WP_042899997.1 5-formyltetrahydrofolate cyclo-ligase -
  QAO20_RS00390 (QAO20_00390) - 71097..72560 (+) 1464 WP_001017867.1 rhomboid family protein -
  QAO20_RS00395 (QAO20_00395) - 72541..72744 (+) 204 WP_000087557.1 YqgQ family protein -
  QAO20_RS00400 (QAO20_00400) - 72741..73727 (+) 987 WP_000161315.1 ROK family glucokinase -
  QAO20_RS00405 (QAO20_00405) - 73727..74056 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  QAO20_RS00410 (QAO20_00410) - 74053..74676 (+) 624 WP_001223003.1 MBL fold metallo-hydrolase -
  QAO20_RS00415 (QAO20_00415) comGA 74728..75702 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  QAO20_RS00420 (QAO20_00420) comGB 75674..76744 (+) 1071 WP_000776415.1 competence type IV pilus assembly protein ComGB Machinery gene
  QAO20_RS00425 (QAO20_00425) comGC 76758..77069 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  QAO20_RS00430 (QAO20_00430) comGD 77047..77493 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  QAO20_RS00435 (QAO20_00435) comGE 77480..77779 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  QAO20_RS00440 (QAO20_00440) comGF 77697..78194 (+) 498 WP_001798585.1 competence type IV pilus minor pilin ComGF Machinery gene
  QAO20_RS00445 (QAO20_00445) - 78291..78437 (+) 147 WP_001789879.1 hypothetical protein -
  QAO20_RS00450 (QAO20_00450) - 78427..78951 (+) 525 WP_001015120.1 shikimate kinase -
  QAO20_RS00455 (QAO20_00455) gcvT 79110..80201 (+) 1092 WP_000093343.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=818797 QAO20_RS00415 WP_000697220.1 74728..75702(+) (comGA) [Staphylococcus aureus strain IT-MSSA50]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=818797 QAO20_RS00415 WP_000697220.1 74728..75702(+) (comGA) [Staphylococcus aureus strain IT-MSSA50]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAATTAT
TAGTTTATATGAAGTTTCAAGCTGGACTTGATGTATCTACACAACAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTCTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1