Detailed information NTG0081584

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HSX14_RS12095
• Genome accession: NZ_AP023189
• Coordinates: 2619754..2620956 (+)
• Length: 400 a.a.
• NCBI ID: WP_373874793.1
• Uniprot ID: A0A6J4E6U1
• Organism: Pseudomonas tohonis strain TUM18999
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2614754..2625956

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HSX14_RS12070 (TUM18999_23970)	-	2615159..2615659 (+)	501	WP_173180314.1	DUF3291 domain-containing protein	-
HSX14_RS12075 (TUM18999_23980)	-	2615682..2616899 (+)	1218	WP_173180315.1	winged helix-turn-helix domain-containing protein	-
HSX14_RS12080 (TUM18999_23990)	-	2617190..2618779 (+)	1590	WP_173180316.1	ABC-F family ATPase	-
HSX14_RS12085 (TUM18999_24000)	-	2618972..2619250 (+)	279	WP_173180317.1	hypothetical protein	-
HSX14_RS12090 (TUM18999_24010)	-	2619318..2619596 (-)	279	WP_044409048.1	peptidylprolyl isomerase	-
HSX14_RS12095 (TUM18999_24020)	pilU	2619754..2620956 (+)	1203	WP_373874793.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HSX14_RS12100 (TUM18999_24030)	-	2621029..2621787 (-)	759	WP_228723578.1	substrate-binding periplasmic protein	-
HSX14_RS12105 (TUM18999_24040)	-	2621863..2622834 (-)	972	WP_173180319.1	DUF4431 domain-containing protein	-
HSX14_RS12110 (TUM18999_24050)	-	2622992..2624947 (-)	1956	WP_173180320.1	acetoacetate--CoA ligase	-

Sequence

Protein

• Length: 400 a.a.
• Molecular weight: 44154.44 Da
• Isoelectric Point: 6.6007

>NTDB_id=81584 HSX14_RS12095 WP_373874793.1 2619754..2620956(+) (pilU) [Pseudomonas tohonis strain TUM18999]
MLKVLASQDGSDLYLSTGAPPCAKFNGVLKPLGTDPLKPGDVARIAESIMDPEQRGEFDRELEMNLAISLPNIGRFRINI
FKQRNEVSIVARNIKLDIPKFEDLKLPEVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIEDPVEYV
HRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRIINFFP
EERRPQLLNDLGNNLKAFVSQRLVRTSDGKRRAAVEVLLGTATISDLIKRGDFSSIKEIMEKSKALGMQTFDQALFDLVV
EGSIQEEEAIKNADSANNLRLKLKLHRDGPGTRPTAAPTPPPAPTPQATPTQDAASWGLELKLEELPEDEPPPEDPGKHY

Nucleotide

• Length: 1203 bp

>NTDB_id=81584 HSX14_RS12095 WP_373874793.1 2619754..2620956(+) (pilU) [Pseudomonas tohonis strain TUM18999]
ATGCTCAAGGTTCTGGCCTCCCAGGATGGCTCGGACCTCTACCTGTCCACCGGTGCGCCGCCCTGCGCCAAGTTCAACGG
CGTGCTCAAGCCGCTGGGCACCGACCCGCTCAAGCCGGGCGACGTGGCGCGTATCGCCGAATCCATCATGGACCCGGAGC
AGCGCGGCGAATTCGACCGCGAGCTGGAGATGAACCTGGCCATCTCCCTGCCCAACATCGGCCGCTTCCGCATCAACATC
TTCAAGCAGCGCAACGAGGTCTCCATCGTCGCGCGCAACATCAAGCTGGATATCCCGAAGTTCGAGGACCTCAAGCTCCC
CGAGGTGCTGCTCAAGGTGGTGATGGAAAAACGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCTCGGGCAAGTCCACCT
CCCTGGCCGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGGCACATCATCACCATCGAGGACCCGGTGGAGTACGTG
CACCGGCACAAGAAATCCATCATCAACCAGCGCGAGGTGGGGGTGGACACCCGCAGCTTCCACGCCGCCCTGAAGAACAC
CCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAACACGCCCTGGCCTTCGCCG
ACACCGGCCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAAGCGCTGGACCGCATCATCAACTTCTTCCCC
GAGGAGCGCCGGCCGCAGCTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTCTCCCAGCGCCTGGTGCGCACCTC
CGACGGCAAGCGCCGCGCGGCGGTGGAAGTGCTGCTGGGCACCGCCACCATCAGCGACCTGATCAAGCGTGGCGACTTCA
GCTCGATCAAGGAAATCATGGAGAAGTCCAAGGCCCTGGGCATGCAGACCTTCGACCAGGCGCTGTTCGACCTGGTGGTG
GAGGGCTCGATCCAGGAAGAGGAAGCGATCAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAAGCTCAAGCTGCACCG
TGACGGCCCCGGCACCCGGCCCACCGCCGCACCCACCCCGCCCCCCGCGCCGACGCCCCAGGCCACCCCGACCCAGGACG
CCGCCAGCTGGGGCCTGGAGCTCAAGCTCGAAGAACTGCCCGAGGACGAGCCGCCGCCGGAAGACCCGGGCAAGCACTAC
TGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (106-266)

3D structure

• AlphaFold DB: A0A6J4E6U1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.866	89.5	0.5
pilU	Acinetobacter baylyi ADP1	53.314	86.75	0.462
pilU	Vibrio cholerae strain A1552	52.326	86	0.45