Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS25_RS09600 Genome accession   NZ_CP121859
Coordinates   1984296..1985405 (+) Length   369 a.a.
NCBI ID   WP_005340356.1    Uniprot ID   -
Organism   Aeromonas veronii strain LOID 15995     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1979296..1990405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS25_RS09570 - 1980197..1980616 (-) 420 WP_120414748.1 DUF4426 domain-containing protein -
  QBS25_RS09575 yggU 1980660..1980962 (-) 303 WP_120414749.1 DUF167 family protein YggU -
  QBS25_RS09580 - 1980962..1981513 (-) 552 WP_005341416.1 YggT family protein -
  QBS25_RS09585 proC 1981525..1982349 (-) 825 WP_279499018.1 pyrroline-5-carboxylate reductase -
  QBS25_RS09590 - 1982492..1983193 (-) 702 WP_279499019.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS25_RS09595 pilT 1983233..1984267 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QBS25_RS09600 pilU 1984296..1985405 (+) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  QBS25_RS09605 yaaA 1985453..1986226 (+) 774 WP_279499020.1 peroxide stress protein YaaA -
  QBS25_RS09610 - 1986421..1986846 (+) 426 WP_005340360.1 hypothetical protein -
  QBS25_RS09615 - 1987206..1987697 (-) 492 WP_279499021.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QBS25_RS09620 arfB 1987730..1988146 (-) 417 WP_111806077.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QBS25_RS09625 glnK 1988348..1988686 (+) 339 WP_005341406.1 P-II family nitrogen regulator -
  QBS25_RS09630 - 1988945..1989952 (+) 1008 WP_005340374.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41076.12 Da        Isoelectric Point: 6.5043

>NTDB_id=814857 QBS25_RS09600 WP_005340356.1 1984296..1985405(+) (pilU) [Aeromonas veronii strain LOID 15995]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=814857 QBS25_RS09600 WP_005340356.1 1984296..1985405(+) (pilU) [Aeromonas veronii strain LOID 15995]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCAGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGGGAAGCGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAAGCGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCTGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGATCTGCAGCTACCCAAAATTCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTTGGCGCCACCGGGG
CGGGCAAATCGACTACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAC
CCGGTGGAGTTCGTCCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCATTCGATGT
GGCGCTGAAAAGTTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGGAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAGGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACTGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACCTTCGATCAAGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGTACCGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36