Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QBS25_RS09595 Genome accession   NZ_CP121859
Coordinates   1983233..1984267 (+) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas veronii strain LOID 15995     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1978233..1989267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS25_RS09565 - 1978899..1980095 (+) 1197 WP_005357935.1 NupC/NupG family nucleoside CNT transporter -
  QBS25_RS09570 - 1980197..1980616 (-) 420 WP_120414748.1 DUF4426 domain-containing protein -
  QBS25_RS09575 yggU 1980660..1980962 (-) 303 WP_120414749.1 DUF167 family protein YggU -
  QBS25_RS09580 - 1980962..1981513 (-) 552 WP_005341416.1 YggT family protein -
  QBS25_RS09585 proC 1981525..1982349 (-) 825 WP_279499018.1 pyrroline-5-carboxylate reductase -
  QBS25_RS09590 - 1982492..1983193 (-) 702 WP_279499019.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS25_RS09595 pilT 1983233..1984267 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QBS25_RS09600 pilU 1984296..1985405 (+) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  QBS25_RS09605 yaaA 1985453..1986226 (+) 774 WP_279499020.1 peroxide stress protein YaaA -
  QBS25_RS09610 - 1986421..1986846 (+) 426 WP_005340360.1 hypothetical protein -
  QBS25_RS09615 - 1987206..1987697 (-) 492 WP_279499021.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QBS25_RS09620 arfB 1987730..1988146 (-) 417 WP_111806077.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QBS25_RS09625 glnK 1988348..1988686 (+) 339 WP_005341406.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=814856 QBS25_RS09595 WP_005340354.1 1983233..1984267(+) (pilT) [Aeromonas veronii strain LOID 15995]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=814856 QBS25_RS09595 WP_005340354.1 1983233..1984267(+) (pilT) [Aeromonas veronii strain LOID 15995]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACATCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCACGT
TTCCGGGTTAACGCCTTCCAGCAGGCGCGCGGTTCGGGCGCGGTATTTCGTACCATCCCCAGCACTGTGCTGAGCCTCGA
AGATCTCGATGCGCCGGAGATCTTCCGCAAGATTGCCGAGTTCCCGCGGGGTCTGGTGTTGGTAACCGGCCCGACCGGTT
CCGGTAAGTCGACCACCCTGGCGGCTATGGTCAATTACATCAACGAGAACTTCCATCACCACATCCTCACCATTGAAGAC
CCCATCGAATTCGTCCACGAGAACAAGCGCTGTCTGGTAAACCAGCGGGAAGTGCACCGGGATACCAAGAGCTTCAGCAA
CGCCCTACGCTCGGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGCGACCTTGAGACCATTCGTTTGG
CCATGACCGCCGCCGAAACTGGCCATCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCGCGGAGAAGGACATGGTGCGCTCCATGCTCTCAGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGTATTGGCGGTGGCCGGGTGGCGGCTCACGAGATCATGATGGGCATTCCGGCGGTGCGTAACCTGATCC
GGGAGGACAAGATTGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGTCGTGGCGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCTGTTGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407