Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   KI219_RS05515 Genome accession   NZ_AP023089
Coordinates   1122592..1123221 (-) Length   209 a.a.
NCBI ID   WP_023855254.1    Uniprot ID   A0A7Z0WXB8
Organism   Bacillus paralicheniformis strain RSC-2     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1117592..1128221
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI219_RS05495 (RSC2_01159) holA 1118151..1119176 (-) 1026 WP_023855252.1 DNA polymerase III subunit delta -
  KI219_RS05500 (RSC2_01160) - 1119406..1119537 (+) 132 WP_003183686.1 YqzM family protein -
  KI219_RS05505 (RSC2_01161) - 1119562..1121949 (-) 2388 WP_023855253.1 DNA internalization-related competence protein ComEC/Rec2 -
  KI219_RS05510 (RSC2_01162) - 1121954..1122523 (-) 570 WP_020452273.1 ComE operon protein 2 -
  KI219_RS05515 (RSC2_01163) comEA 1122592..1123221 (-) 630 WP_023855254.1 helix-hairpin-helix domain-containing protein Machinery gene
  KI219_RS05520 (RSC2_01164) comER 1123304..1124125 (+) 822 WP_020452275.1 late competence protein ComER -
  KI219_RS05525 (RSC2_01165) - 1124196..1124951 (-) 756 WP_023855255.1 class I SAM-dependent methyltransferase -
  KI219_RS05530 (RSC2_01166) rsfS 1124948..1125304 (-) 357 WP_023855256.1 ribosome silencing factor -
  KI219_RS05535 (RSC2_01167) yqeK 1125318..1125881 (-) 564 WP_023855257.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  KI219_RS05540 (RSC2_01168) - 1125871..1126440 (-) 570 WP_025811125.1 nicotinate-nucleotide adenylyltransferase -
  KI219_RS05545 (RSC2_01169) yhbY 1126459..1126749 (-) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  KI219_RS05550 (RSC2_01170) aroE 1126743..1127579 (-) 837 WP_020452280.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22594.42 Da        Isoelectric Point: 4.6492

>NTDB_id=81346 KI219_RS05515 WP_023855254.1 1122592..1123221(-) (comEA) [Bacillus paralicheniformis strain RSC-2]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSGLSIPEEASAQTFDKKEEVKREKNAGEEEVIIDLKGAVKNPGV
YQMKEGDRVHDALKKAGGTEKKADQKQINLAAVLRDGMVLYIPFEGEEAAGSLSEADSRADGSSSDIVNINTASSEELQT
IPGIGPSKAEAVVEYREENGMFQTIEDITNVSGIGEKSFERIKSSITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=81346 KI219_RS05515 WP_023855254.1 1122592..1123221(-) (comEA) [Bacillus paralicheniformis strain RSC-2]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCGGCGGGAGGTGTGGCACTCGTCTTGATCATCAGCGCTGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCCGGCCTCTCCATTCCTGAAGAGGCTTCTGCACAGACTTTTGACAAGAAAG
AGGAGGTGAAGCGGGAAAAAAACGCGGGGGAAGAGGAGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAGGAGGGAGACAGGGTGCACGATGCATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGACCAAAAGCA
AATTAATCTGGCAGCCGTTTTGCGGGACGGTATGGTTCTTTACATTCCATTTGAAGGGGAAGAGGCCGCTGGCTCTCTTT
CGGAAGCGGACTCAAGAGCAGACGGCAGCTCAAGTGATATCGTCAATATCAATACGGCTTCCTCTGAGGAGCTTCAGACG
ATTCCCGGGATCGGTCCTTCAAAAGCGGAAGCGGTAGTCGAATACCGCGAGGAGAACGGAATGTTTCAGACGATTGAAGA
CATAACAAACGTTTCGGGAATTGGAGAGAAGTCTTTTGAAAGAATAAAATCTTCCATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(69-122)

Helix-hairpin-helix motif (HHH_3)

(145-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z0WXB8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

52.336

100

0.536
  comEA Staphylococcus aureus MW2

35.909

100

0.378
  comEA Staphylococcus aureus N315

35.909

100

0.378

Multiple sequence alignment