Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   P8F79_RS07100 Genome accession   NZ_CP121172
Coordinates   1421221..1422123 (+) Length   300 a.a.
NCBI ID   WP_048539722.1    Uniprot ID   -
Organism   Mammaliicoccus sciuri strain PC04     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1416221..1427123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P8F79_RS07060 (P8F79_07055) - 1416307..1416867 (+) 561 WP_048539717.1 5-formyltetrahydrofolate cyclo-ligase -
  P8F79_RS07065 (P8F79_07060) - 1416860..1418308 (+) 1449 WP_048539718.1 rhomboid family intramembrane serine protease -
  P8F79_RS07070 (P8F79_07065) - 1418313..1418504 (+) 192 WP_078099923.1 YqgQ family protein -
  P8F79_RS07075 (P8F79_07070) - 1418501..1419472 (+) 972 WP_048539719.1 ROK family glucokinase -
  P8F79_RS07080 (P8F79_07075) - 1419472..1419786 (+) 315 WP_048539720.1 MTH1187 family thiamine-binding protein -
  P8F79_RS07085 (P8F79_07080) - 1419815..1420054 (-) 240 WP_016911885.1 DUF2759 domain-containing protein -
  P8F79_RS07090 (P8F79_07085) - 1420152..1420784 (+) 633 WP_048539721.1 MBL fold metallo-hydrolase -
  P8F79_RS07095 (P8F79_07090) - 1420819..1421067 (-) 249 WP_025904960.1 DUF2626 family protein -
  P8F79_RS07100 (P8F79_07095) comGA 1421221..1422123 (+) 903 WP_048539722.1 competence type IV pilus ATPase ComGA Machinery gene
  P8F79_RS07105 (P8F79_07100) comGB 1422113..1423153 (+) 1041 WP_075577987.1 competence type IV pilus assembly protein ComGB -
  P8F79_RS07110 (P8F79_07105) comGC 1423168..1423485 (+) 318 WP_037588275.1 competence type IV pilus major pilin ComGC Machinery gene
  P8F79_RS07115 (P8F79_07110) comGD 1423463..1423912 (+) 450 WP_048539818.1 competence type IV pilus minor pilin ComGD -
  P8F79_RS07120 (P8F79_07115) comGE 1423896..1424177 (+) 282 WP_048539723.1 competence type IV pilus minor pilin ComGE -
  P8F79_RS07125 (P8F79_07120) - 1424152..1424586 (+) 435 WP_048539724.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  P8F79_RS07130 (P8F79_07125) - 1424821..1425309 (+) 489 WP_048539725.1 shikimate kinase -
  P8F79_RS07135 (P8F79_07130) gcvT 1425502..1426590 (+) 1089 WP_048539726.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34442.27 Da        Isoelectric Point: 6.1709

>NTDB_id=810841 P8F79_RS07100 WP_048539722.1 1421221..1422123(+) (comGA) [Mammaliicoccus sciuri strain PC04]
MEKLFKSIINDAVNSNASDIHFVPLNDDKVFVQMRINGDNTPYDLDLDFKLYSRLLIYMKFIAHLDVSERNKAQSGQYLY
EHDIHYYLRISTIPISLGIESCSIRITPQHFNQSQVKSEHQSYNVLQNRMYQSEGLFLFTGPTGSGKTTLMYELMYALKR
EKNKHIITIEDPVEQTLNGIVQVSVNEKANITYQQSFKAILRCDPDVIMIGEIRDDVTAKQVIQASLSGHLVLSTMHASD
TIGAIHRLIELGVSVEQIKQGISCITNQRLVKKNDETRTLNMSYIFKDDIINYMENDYET

Nucleotide


Download         Length: 903 bp        

>NTDB_id=810841 P8F79_RS07100 WP_048539722.1 1421221..1422123(+) (comGA) [Mammaliicoccus sciuri strain PC04]
ATGGAGAAATTATTTAAGTCAATTATTAACGATGCTGTCAATTCAAATGCGAGTGATATTCACTTTGTACCTTTAAATGA
TGATAAAGTCTTTGTTCAAATGAGGATAAATGGTGATAATACACCTTATGATTTAGATCTAGACTTTAAATTGTATAGTA
GGCTACTCATTTATATGAAATTTATCGCTCATCTTGATGTAAGTGAACGTAATAAAGCACAAAGCGGTCAATATTTATAT
GAACATGACATTCATTACTACTTAAGAATTTCTACGATACCTATCAGTTTAGGAATAGAATCTTGTTCCATTAGAATTAC
CCCTCAACATTTTAATCAATCACAAGTGAAATCAGAGCATCAATCATATAACGTTTTACAAAATAGAATGTATCAATCAG
AAGGGCTATTTCTATTTACGGGTCCAACTGGATCAGGTAAGACGACATTAATGTATGAATTGATGTACGCTTTAAAGAGA
GAAAAGAACAAACATATTATTACGATTGAAGACCCTGTAGAACAAACGTTAAATGGCATCGTTCAAGTTTCAGTCAATGA
AAAAGCTAACATAACTTATCAACAATCCTTTAAAGCCATTTTAAGATGTGATCCTGACGTTATTATGATTGGAGAGATAA
GAGACGATGTTACTGCTAAGCAAGTTATTCAAGCTAGTTTAAGTGGTCATTTAGTACTTTCAACAATGCACGCGAGTGAT
ACAATTGGGGCAATCCATCGACTAATAGAACTAGGTGTATCAGTCGAACAAATTAAACAAGGTATTTCATGTATTACAAA
CCAACGTCTTGTGAAGAAAAATGATGAAACAAGAACTTTAAATATGTCTTATATTTTTAAAGATGACATTATTAACTATA
TGGAGAATGATTATGAAACGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

52.857

93.333

0.493
  comGA Staphylococcus aureus N315

52.857

93.333

0.493
  comGA Bacillus subtilis subsp. subtilis str. 168

40.58

92

0.373