Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ROT04_RS27410 Genome accession   NZ_CP135174
Coordinates   5878462..5879085 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain PARM_L1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5873462..5884085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ROT04_RS27390 (ROT04_27390) aroB 5874057..5875163 (-) 1107 WP_003112148.1 3-dehydroquinate synthase -
  ROT04_RS27395 (ROT04_27395) aroK 5875213..5875731 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ROT04_RS27400 (ROT04_27400) pilQ 5875743..5877887 (-) 2145 WP_003095835.1 type 4a pilus secretin PilQ Machinery gene
  ROT04_RS27405 (ROT04_27405) comL/pilP 5877941..5878465 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  ROT04_RS27410 (ROT04_27410) pilO 5878462..5879085 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  ROT04_RS27415 (ROT04_27415) pilN 5879082..5879678 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  ROT04_RS27420 (ROT04_27420) pilM 5879678..5880742 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ROT04_RS27425 (ROT04_27425) - 5880927..5883398 (+) 2472 WP_058355269.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=810397 ROT04_RS27410 WP_003103268.1 5878462..5879085(-) (pilO) [Pseudomonas aeruginosa strain PARM_L1]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=810397 ROT04_RS27410 WP_003103268.1 5878462..5879085(-) (pilO) [Pseudomonas aeruginosa strain PARM_L1]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401