Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   QN339_RS00520 Genome accession   NZ_CP135104
Coordinates   98208..98663 (+) Length   151 a.a.
NCBI ID   WP_410534293.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain MA15     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 93208..103663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QN339_RS00505 (QN339_00505) - 95765..96130 (+) 366 WP_000285373.1 DUF1033 family protein -
  QN339_RS00510 (QN339_00510) comGA 96303..97274 (+) 972 WP_000250805.1 competence type IV pilus ATPase ComGA Machinery gene
  QN339_RS00515 (QN339_00515) comGB 97168..98211 (+) 1044 WP_223295889.1 competence type IV pilus assembly protein ComGB Machinery gene
  QN339_RS00520 (QN339_00520) comGF 98208..98663 (+) 456 WP_410534293.1 competence type IV pilus minor pilin ComGF Machinery gene
  QN339_RS00525 (QN339_00525) comGG 98641..99012 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG Machinery gene
  QN339_RS00530 (QN339_00530) comGH 99127..100101 (+) 975 WP_001008574.1 class I SAM-dependent methyltransferase Machinery gene
  QN339_RS00535 (QN339_00535) - 100133..101326 (+) 1194 WP_000047534.1 acetate kinase -
  QN339_RS00540 (QN339_00540) - 101478..101684 (+) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  QN339_RS00545 (QN339_00545) - 101743..101880 (+) 138 WP_001865900.1 hypothetical protein -
  QN339_RS00550 (QN339_00550) - 101921..102376 (+) 456 WP_000905673.1 hypothetical protein -
  QN339_RS00555 (QN339_00555) - 102445..103110 (+) 666 WP_000008113.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 17508.53 Da        Isoelectric Point: 9.9510

>NTDB_id=810003 QN339_RS00520 WP_410534293.1 98208..98663(+) (comGF) [Streptococcus agalactiae strain MA15]
MKNLLLKCKDKKVKAFTLLKCLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQNK
LYLRKQDKIVTFGKSNKDDFRKTGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET

Nucleotide


Download         Length: 456 bp        

>NTDB_id=810003 QN339_RS00520 WP_410534293.1 98208..98663(+) (comGF) [Streptococcus agalactiae strain MA15]
ATGAAAAATTTATTGTTAAAATGTAAGGATAAGAAGGTTAAAGCATTTACACTTTTAAAATGTTTGGTAGCATTGGTTAC
AATCACAGGAGCTTTACTAGTTTATCAAGGACTGACAAAATTGTTGGCTCAACAGATAGTAGTGATGTCTTCTTCCAGTC
AGTCTGAATGGGTGTTATTAACTCAGCAACTAAATGCAGAATTTGAAGGCGCTCATCTGGAATATTTAAGACAGAACAAA
CTTTATTTACGTAAGCAAGATAAGATTGTAACCTTTGGCAAATCTAATAAAGATGATTTCCGTAAGACAGGTTATGATGG
TCGAGGTTATCAACCAATGGTTTATGGGTTAGACAATTGTCAAATGAGTCAGACCAAAAGTATGGTAAAACTTGTTTTTT
ATTTTAAGGACGGGTTAAAAAGGACATTTTACTATGATTTTAAAGAAGAAACTTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Streptococcus mutans UA159

47.857

92.715

0.444
  comGF Streptococcus mutans UA140

47.857

92.715

0.444
  comGF/cglF Streptococcus pneumoniae Rx1

41.333

99.338

0.411
  comGF/cglF Streptococcus pneumoniae D39

41.333

99.338

0.411
  comGF/cglF Streptococcus pneumoniae R6

41.333

99.338

0.411
  comGF/cglF Streptococcus pneumoniae TIGR4

41.333

99.338

0.411
  comGF/cglF Streptococcus mitis SK321

43.478

91.391

0.397
  comGF/cglF Streptococcus mitis NCTC 12261

42.754

91.391

0.391
  comGF Lactococcus lactis subsp. cremoris KW2

39.855

91.391

0.364