Detailed information NTG0809392

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLNCDO1820_RS09355
• Genome accession: NZ_CP120927
• Coordinates: 1836164..1836811 (-)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis subsp. lactis strain NCDO1820
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1831164..1841811

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLNCDO1820_RS09330 (LLNCDO1820_09320)	atpF	1831234..1831740 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
LLNCDO1820_RS09335 (LLNCDO1820_09325)	atpB	1831755..1832468 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LLNCDO1820_RS09340 (LLNCDO1820_09330)	-	1832513..1832728 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LLNCDO1820_RS09345 (LLNCDO1820_09335)	-	1832912..1833688 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
LLNCDO1820_RS09350 (LLNCDO1820_09340)	comEC	1833973..1836183 (-)	2211	WP_010906130.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LLNCDO1820_RS09355 (LLNCDO1820_09345)	comEA	1836164..1836811 (-)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
LLNCDO1820_RS09360 (LLNCDO1820_09350)	-	1836871..1838232 (-)	1362	WP_021214597.1	ABC transporter permease	-
LLNCDO1820_RS09365 (LLNCDO1820_09355)	-	1838229..1839161 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LLNCDO1820_RS09370 (LLNCDO1820_09360)	-	1839266..1839664 (-)	399	WP_004255236.1	hypothetical protein	-
LLNCDO1820_RS09375 (LLNCDO1820_09365)	-	1839768..1840331 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LLNCDO1820_RS09380 (LLNCDO1820_09370)	-	1840504..1841682 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=809392 LLNCDO1820_RS09355 WP_010906131.1 1836164..1836811(-) (comEA) [Lactococcus lactis subsp. lactis strain NCDO1820]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=809392 LLNCDO1820_RS09355 WP_010906131.1 1836164..1836811(-) (comEA) [Lactococcus lactis subsp. lactis strain NCDO1820]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386