Detailed information NTG0809191

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: P6N30_RS22060
• Genome accession: NZ_CP120897
• Coordinates: 4589118..4589915 (+)
• Length: 265 a.a.
• NCBI ID: WP_015585126.1
• Uniprot ID: A0A855FAW5
• Organism: Raoultella ornithinolytica strain GY22PK019
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4584118..4594915

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P6N30_RS22025 (P6N30_22020)	gspH	4584238..4584756 (+)	519	WP_015585120.1	type II secretion system minor pseudopilin GspH	-
P6N30_RS22030 (P6N30_22025)	gspI	4584753..4585118 (+)	366	WP_004858044.1	type II secretion system minor pseudopilin GspI	-
P6N30_RS22035 (P6N30_22030)	gspJ	4585130..4585714 (+)	585	WP_015585121.1	type II secretion system minor pseudopilin GspJ	-
P6N30_RS22040 (P6N30_22035)	gspK	4585711..4586697 (+)	987	WP_015585122.1	type II secretion system minor pseudopilin GspK	-
P6N30_RS22045 (P6N30_22040)	gspL	4586684..4587886 (+)	1203	WP_204111659.1	type II secretion system protein GspL	-
P6N30_RS22050 (P6N30_22045)	gspM	4587879..4588373 (+)	495	WP_064357507.1	type II secretion system protein GspM	-
P6N30_RS22055 (P6N30_22050)	-	4588363..4589103 (+)	741	WP_015585125.1	type II secretion system protein N	-
P6N30_RS22060 (P6N30_22055)	pilD	4589118..4589915 (+)	798	WP_015585126.1	A24 family peptidase	Machinery gene
P6N30_RS22065 (P6N30_22060)	hrpB	4589936..4592365 (-)	2430	WP_044348317.1	ATP-dependent helicase HrpB	-
P6N30_RS22070 (P6N30_22065)	thpR	4592438..4592974 (+)	537	WP_004858022.1	RNA 2',3'-cyclic phosphodiesterase	-
P6N30_RS22075 (P6N30_22070)	sfsA	4592974..4593690 (+)	717	WP_004858020.1	DNA/RNA nuclease SfsA	-
P6N30_RS22080 (P6N30_22075)	dksA	4593855..4594310 (+)	456	WP_004858018.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29285.93 Da
• Isoelectric Point: 8.4762

>NTDB_id=809191 P6N30_RS22060 WP_015585126.1 4589118..4589915(+) (pilD) [Raoultella ornithinolytica strain GY22PK019]
MTLLSAFSLQFPLLWGGLLFIFGLTFGSFFNVVIYRLPLMMQQEESARFNLCVPASFCPQCQRPIIWRDNIPLLSYLSLK
GRARCCQAPISQRYPLTELASGLLFVLAGYLLTPGLPLLGGLILLSTLLVLAIIDGQTQLLPDRLTLPLLWAGLLFNLNG
TFVPLSEAVIGAMTGYLSLWTVYWLFRLLTGKEALGYGDFKLLAALGAWSGWQILPQTLLCASASGLIWTLLQRRITLQS
LDQPLAFGPWLALAGSGLFLWTQTL

Nucleotide

• Length: 798 bp

>NTDB_id=809191 P6N30_RS22060 WP_015585126.1 4589118..4589915(+) (pilD) [Raoultella ornithinolytica strain GY22PK019]
ATGACGCTACTGAGCGCATTTTCGTTGCAGTTCCCCCTGTTATGGGGCGGATTGTTGTTTATTTTCGGCCTGACGTTTGG
CAGCTTTTTTAACGTGGTTATCTACCGTTTGCCGCTGATGATGCAGCAGGAAGAGTCCGCACGATTCAACCTGTGCGTTC
CCGCCTCCTTCTGCCCCCAGTGCCAGCGGCCCATCATCTGGCGGGATAATATTCCGCTGCTCAGCTATCTCTCACTCAAA
GGGCGCGCCCGCTGCTGCCAGGCGCCCATTTCTCAGCGCTATCCGCTGACGGAACTGGCCAGCGGACTGCTCTTTGTGTT
GGCCGGCTATCTGCTGACGCCGGGATTACCGCTGCTGGGGGGCCTGATCCTGCTCTCCACGCTGCTGGTACTGGCGATCA
TCGACGGCCAGACTCAGCTCCTGCCGGATCGGCTGACTCTGCCGCTGCTGTGGGCCGGTCTGCTGTTTAATCTCAACGGC
ACGTTTGTCCCGCTCAGCGAAGCGGTGATCGGCGCCATGACGGGCTATTTATCACTGTGGACGGTTTACTGGCTGTTTCG
CCTGCTGACCGGCAAAGAGGCGCTAGGATACGGTGATTTTAAGCTGCTGGCGGCACTCGGCGCATGGTCCGGCTGGCAGA
TCCTGCCGCAGACGCTGCTGTGCGCCTCGGCCAGCGGCCTGATCTGGACCTTGCTGCAACGGCGGATCACGCTCCAGTCG
CTGGATCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCAGCGGCCTGTTTCTGTGGACTCAGACGCTGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (122-230)

3D structure

• AlphaFold DB: A0A855FAW5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.529	100	0.498
pilD	Vibrio campbellii strain DS40M4	45.221	100	0.464
pilD	Neisseria gonorrhoeae MS11	46.614	94.717	0.442
pilD	Acinetobacter baumannii D1279779	45.276	95.849	0.434
pilD	Acinetobacter nosocomialis M2	44.186	97.358	0.43