Detailed information NTG0809059

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P4833_RS03225
• Genome accession: NZ_CP120871
• Coordinates: 700090..701238 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PACL
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 695090..706238

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P4833_RS03200 (P4833_03180)	-	696241..696537 (-)	297	WP_077144761.1	DUF167 domain-containing protein	-
P4833_RS03205 (P4833_03185)	-	696534..697127 (-)	594	WP_003084544.1	YggT family protein	-
P4833_RS03210 (P4833_03190)	proC	697138..697959 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
P4833_RS03215 (P4833_03195)	-	697971..698663 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
P4833_RS03220 (P4833_03200)	pilT	698878..699912 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
P4833_RS03225 (P4833_03205)	pilU	700090..701238 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
P4833_RS03230 (P4833_03210)	czcD	701246..702145 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
P4833_RS03235 (P4833_03215)	-	702299..702703 (+)	405	WP_003084560.1	NINE protein	-
P4833_RS03240 (P4833_03220)	-	702946..704319 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
P4833_RS03245 (P4833_03225)	-	704316..705500 (+)	1185	WP_003117897.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=809059 P4833_RS03225 WP_003100959.1 700090..701238(+) (pilU) [Pseudomonas aeruginosa strain PACL]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=809059 P4833_RS03225 WP_003100959.1 700090..701238(+) (pilU) [Pseudomonas aeruginosa strain PACL]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAAGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382