Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   P4829_RS13025 Genome accession   NZ_CP120846
Coordinates   2583135..2584205 (-) Length   356 a.a.
NCBI ID   WP_063638548.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain MBLB1156     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2578135..2589205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4829_RS12980 (P4829_12980) tapA 2578670..2579371 (-) 702 WP_063638542.1 amyloid fiber anchoring/assembly protein TapA -
  P4829_RS12985 (P4829_12985) - 2579633..2579956 (+) 324 WP_063638543.1 DUF3889 domain-containing protein -
  P4829_RS12990 (P4829_12990) - 2580003..2580182 (-) 180 WP_003325435.1 YqzE family protein -
  P4829_RS12995 (P4829_12995) comGG 2580252..2580626 (-) 375 WP_063638544.1 competence type IV pilus minor pilin ComGG Machinery gene
  P4829_RS13000 (P4829_13000) comGF 2580627..2581022 (-) 396 WP_309484978.1 competence type IV pilus minor pilin ComGF Machinery gene
  P4829_RS13005 (P4829_13005) comGE 2581036..2581383 (-) 348 WP_063638545.1 competence type IV pilus minor pilin ComGE Machinery gene
  P4829_RS13010 (P4829_13010) comGD 2581367..2581807 (-) 441 WP_063638546.1 competence type IV pilus minor pilin ComGD Machinery gene
  P4829_RS13015 (P4829_13015) comGC 2581797..2582105 (-) 309 WP_087941811.1 competence type IV pilus major pilin ComGC Machinery gene
  P4829_RS13020 (P4829_13020) comGB 2582111..2583148 (-) 1038 WP_063638547.1 competence type IV pilus assembly protein ComGB Machinery gene
  P4829_RS13025 (P4829_13025) comGA 2583135..2584205 (-) 1071 WP_063638548.1 competence type IV pilus ATPase ComGA Machinery gene
  P4829_RS13030 (P4829_13030) - 2584607..2585560 (-) 954 WP_061572822.1 magnesium transporter CorA family protein -
  P4829_RS13035 (P4829_13035) - 2585672..2587030 (+) 1359 WP_063638549.1 hemolysin family protein -
  P4829_RS13040 (P4829_13040) - 2587097..2587948 (-) 852 WP_010789205.1 STAS domain-containing protein -
  P4829_RS13050 (P4829_13050) - 2588207..2588587 (-) 381 WP_003325422.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  P4829_RS13055 (P4829_13055) - 2588824..2589069 (+) 246 WP_003325421.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40457.76 Da        Isoelectric Point: 9.4624

>NTDB_id=808857 P4829_RS13025 WP_063638548.1 2583135..2584205(-) (comGA) [Bacillus atrophaeus strain MBLB1156]
MDTIEKTSRTLIEEAYSVKASDIHIVPREQDALIHFRIDHALLKKRTMKKEECVRLISHFKFLSAMDIGERRKPQSGSLS
LKLKKGTVYLRMSTLPTINDESLVIRVLPQHNIPAIERLSLFPKTGATLLSFLKHSHGLIIFTGPTGSGKSTTLYSLVQY
AKKHFNRNIITLEDPVETRDQDVLQVQVNEKAGITYSAGLKAILRHDPDMIVLGEIRDAETAKIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGVNMNEIEQTAIAIAAQRLVDLTCPFCENGCSSVYCRITRDVRRATIYELLYGKNLQQCIQEARGE
RANYQYRTLRQIIRKGIALGYLTTNNYDRWVYHEED

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=808857 P4829_RS13025 WP_063638548.1 2583135..2584205(-) (comGA) [Bacillus atrophaeus strain MBLB1156]
TTGGATACGATAGAAAAAACTAGCAGGACATTAATTGAAGAGGCATATTCCGTGAAGGCCTCGGATATTCACATTGTACC
AAGGGAGCAAGATGCTTTGATCCATTTTCGGATTGACCATGCGTTGCTGAAGAAGCGGACAATGAAAAAGGAAGAGTGCG
TTCGGCTCATTTCCCATTTTAAATTTCTGTCAGCCATGGATATAGGAGAAAGGCGGAAGCCGCAAAGTGGTTCACTTTCT
TTAAAGCTTAAAAAAGGGACAGTTTATCTCAGAATGTCGACACTTCCTACGATTAATGATGAAAGCCTCGTGATCAGAGT
TTTGCCTCAGCATAATATTCCTGCGATCGAAAGACTGTCCCTGTTCCCAAAGACAGGCGCCACGCTGCTATCCTTTTTAA
AGCACTCCCATGGCCTCATTATTTTTACCGGTCCGACTGGCTCAGGGAAATCTACCACCCTATATTCACTGGTTCAATAT
GCAAAAAAGCATTTCAACCGAAATATTATCACTCTCGAAGATCCTGTGGAAACTAGAGATCAGGATGTTTTGCAGGTACA
GGTTAATGAAAAAGCCGGCATTACGTACTCAGCGGGCCTGAAGGCGATTTTGCGCCATGATCCAGACATGATTGTTCTGG
GGGAAATCAGGGATGCTGAAACAGCTAAAATCGCCGTGCGGGCTGCTATGACAGGCCATTTGGTATTAACAAGCCTGCAC
ACAAGAGATGCCAAGGGCGCCATTTACAGACTGCTTGAATTCGGAGTAAATATGAATGAAATAGAGCAAACAGCGATTGC
GATAGCAGCCCAGCGATTAGTCGACTTAACCTGTCCATTTTGTGAAAACGGCTGTTCCTCTGTTTATTGCCGGATTACAC
GTGATGTAAGGCGAGCGACCATTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATACAAGAAGCTAGAGGAGAA
CGGGCCAATTATCAGTACCGAACCTTACGGCAGATTATCAGAAAAGGAATCGCGCTTGGCTATCTAACGACGAATAATTA
CGACCGGTGGGTTTATCATGAAGAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

87.921

100

0.879